New cytotoxic dammarane type saponins from Ziziphus spina-christi

Abeer H Elmaidomy; Amr El Zawily; Aliasger K Salem; Faisal H Altemani; Naseh A Algehainy; Abdullah H Altemani; Mostafa E Rateb; Usama Ramadan Abdelmohsen; Nourhan Hisham Shady

doi:10.1038/s41598-023-46841-2

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New cytotoxic dammarane type saponins from Ziziphus spina-christi

Journal article

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New cytotoxic dammarane type saponins from Ziziphus spina-christi

Abeer H Elmaidomy, Amr El Zawily, Aliasger K Salem, Faisal H Altemani, Naseh A Algehainy, Abdullah H Altemani, Mostafa E Rateb, Usama Ramadan Abdelmohsen and Nourhan Hisham Shady

Scientific reports, Vol.13(1), 20612

11/23/2023

DOI: 10.1038/s41598-023-46841-2

PMCID: PMC10667233

PMID: 37996449

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Abstract

Cancer is the world's second-leading cause of death. Drug development efforts frequently focus on medicinal plants since they are a valuable source of anticancer medications. A phytochemical investigation of the edible Ziziphus spina-christi (F. Rhamnaceae) leaf extract afforded two new dammarane type saponins identified as christinin E and F (1, 2), along with the known compound christinin A (3). Different cancer cell lines, such as lung cancer (A549), glioblastoma (U87), breast cancer (MDA-MB-231), and colorectal carcinoma (CT-26) cell lines, were used to investigate the extracted compounds' cytotoxic properties. Our findings showed significant effects on all the tested cell lines at varying concentrations (1, 5, 10, and 20 µg/mL). The three compounds exhibited potent activity at low concentrations (< 10 μg/mL), as evidenced by their low IC50 values. To further investigate the complex relationships between these identified cancer-relevant biological targets and to identify critical targets in the pathogenesis of the disease, we turned to network pharmacology and in silico-based investigations. Following this, in silico-based analysis (e.g., inverse docking, ΔG calculation, and molecular dynamics simulation) was performed on the structures of the isolated compounds to identify additional potential targets for these compounds and their likely interactions with various signalling pathways relevant to this disease. Based on our findings, Z. spina-christi's compounds showed promise as potential anti-cancer therapeutic leads in the future.

Lung Cancer

Molecular Dynamics

Signal Transduction

Cancer

Colorectal carcinoma

Cytotoxicity

Drug development

Glioblastoma

Investigations

Medicinal plants

Plant extracts

Saponins

Tumor cell lines

Ziziphus spina-christi

Details

Title: Subtitle: New cytotoxic dammarane type saponins from Ziziphus spina-christi
Creators: Abeer H Elmaidomy - Beni-Suef University
Amr El Zawily - University of Iowa
Aliasger K Salem - University of Iowa
Faisal H Altemani - University of Tabuk
Naseh A Algehainy - University of Tabuk
Abdullah H Altemani - University of Tabuk
Mostafa E Rateb - University of the West of Scotland
Usama Ramadan Abdelmohsen
Nourhan Hisham Shady - Minia University
Resource Type: Journal article
Publication Details: Scientific reports, Vol.13(1), 20612
DOI: 10.1038/s41598-023-46841-2
PMID: 37996449
PMCID: PMC10667233
NLM abbreviation: Sci Rep
eISSN: 2045-2322
Publisher: Nature Publishing Group
Language: English
Date published: 11/23/2023
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Pharmaceutical Sciences and Experimental Therapeutics; Biology; Craniofacial Anomalies Research Center; Dental Research; Chemical and Biochemical Engineering
Record Identifier: 9984516957702771

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