New insights into the pleiotropic drug resistance network from genome-wide characterization of the YRR1 transcription factor regulation system

Stéphane Le Crom; Frédéric Devaux; Philippe Marc; Xiaoting Zhang; W Scott Moye-Rowley; Claude Jacq

doi:10.1128/mcb.22.8.2642-2649.2002

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New insights into the pleiotropic drug resistance network from genome-wide characterization of the YRR1 transcription factor regulation system

Journal article

Open access

Peer reviewed

New insights into the pleiotropic drug resistance network from genome-wide characterization of the YRR1 transcription factor regulation system

Stéphane Le Crom, Frédéric Devaux, Philippe Marc, Xiaoting Zhang, W Scott Moye-Rowley and Claude Jacq

Molecular and cellular biology, Vol.22(8), pp.2642-2649

04/01/2002

DOI: 10.1128/mcb.22.8.2642-2649.2002

PMCID: PMC133742

PMID: 11909958

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Abstract

Yrr1p is a recently described Zn(2)Cys(6) transcription factor involved in the pleiotropic drug resistance (PDR) phenomenon. It is controlled in a Pdr1p-dependent manner and is autoregulated. We describe here a new genome-wide approach to characterization of the set of genes directly regulated by Yrr1p. We found that the time-course production of an artificial chimera protein containing the DNA-binding domain of Yrr1p activated the 15 genes that are also up-regulated by a gain-of-function mutant of Yrr1p. Gel mobility shift assays showed that the promoters of the genes AZR1, FLR1, SNG1, YLL056C, YLR346C, and YPL088W interacted with Yrr1p. The putative consensus Yrr1p binding site deduced from these experiments, (T/A)CCG(C/T)(G/T)(G/T)(A/T)(A/T), is strikingly similar to the PDR element binding site sequence recognized by Pdr1p and Pdr3p. The minor differences between these sequences are consistent with Yrr1p and Pdr1p and Pdr3p having different sets of target genes. According to these data, some target genes are directly regulated by Pdr1p and Pdr3p or by Yrr1p, whereas some genes are indirectly regulated by the activation of Yrr1p. Some genes, such as YOR1, SNQ2, and FLR1, are clearly directly controlled by both classes of transcription factor, suggesting an important role for the corresponding membrane proteins.

Genomics

Life Sciences

Amino Acid Sequence

Base Sequence

Binding Sites

Biochemistry, Molecular Biology

Consensus Sequence

DNA, Fungal

Drug Resistance, Multiple, Fungal

Gene Expression Profiling

Gene Expression Regulation, Fungal

Genome, Fungal

Models, Biological

Molecular Sequence Data

Oligonucleotide Array Sequence Analysis

Promoter Regions, Genetic

Quantitative Methods

Recombinant Fusion Proteins

Saccharomyces cerevisiae

Sequence Homology, Amino Acid

Transcription Factors

Details

Title: Subtitle: New insights into the pleiotropic drug resistance network from genome-wide characterization of the YRR1 transcription factor regulation system
Creators: Stéphane Le Crom - Laboratoire de Génétique Moléculaire, CNRS UMR 8541, Ecole Normale Supérieure, 75230 Paris Cedex 05, France
Frédéric Devaux - Laboratoire de Génétique Moléculaire, CNRS UMR 8541, Ecole Normale Supérieure, 75230 Paris Cedex 05, France
Philippe Marc - Laboratoire de Génétique Moléculaire, CNRS UMR 8541, Ecole Normale Supérieure, 75230 Paris Cedex 05, France
Xiaoting Zhang - University of Iowa
W Scott Moye-Rowley - University of Iowa
Claude Jacq - Laboratoire de Génétique Moléculaire, CNRS UMR 8541, Ecole Normale Supérieure, 75230 Paris Cedex 05, France
Resource Type: Journal article
Publication Details: Molecular and cellular biology, Vol.22(8), pp.2642-2649
Publisher: American Society for Microbiology
DOI: 10.1128/mcb.22.8.2642-2649.2002
PMID: 11909958
PMCID: PMC133742
ISSN: 0270-7306
eISSN: 1098-5549
Language: English
Date published: 04/01/2002
Academic Unit: Molecular Physiology and Biophysics; Internal Medicine
Record Identifier: 9984297597302771

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