Newly synthesized calsequestrin, destined for the sarcoplasmic reticulum, is contained in early/intermediate Golgi-derived clathrin-coated vesicles

Kathleen Thomas; J NAVARRO; Robin J. J Benson; Kevin P Campbell; R. L ROTUNDO; R. E FINE

doi:10.1016/S0021-9258(18)94042-1

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Newly synthesized calsequestrin, destined for the sarcoplasmic reticulum, is contained in early/intermediate Golgi-derived clathrin-coated vesicles

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Newly synthesized calsequestrin, destined for the sarcoplasmic reticulum, is contained in early/intermediate Golgi-derived clathrin-coated vesicles

Kathleen Thomas, J NAVARRO, Robin J. J Benson, Kevin P Campbell, R. L ROTUNDO and R. E FINE

The Journal of biological chemistry, Vol.264(6), pp.3140-3145

1989

DOI: 10.1016/S0021-9258(18)94042-1

PMID: 2563378

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Abstract

We have examined the possible role of clathrin-coated vesicles (CVs) in the genesis of the sarcoplasmic reticulum (SR) in developing chick skeletal myotubes. Calsequestrin (CSQ) a luminal Ca2+ binding protein of the terminal SR cisternae, is contained within the vesicle lumen of skeletal muscle CVs in substantial amounts, approximately four molecules/CV. Employing 3-day cultures of chick skeletal myotubes we demonstrate that after a 30-min labeling with [35S]methionine and cysteine, radioactivity in CSQ remains high in the CVs 45 min later and then declines, while labeled CSQ in the SR continues to rise. No CSQ appears to be secreted. All of the CSQ in both the CVs and SR is sensitive to the activity of endoglycosidase H, and a significant fraction also binds to wheat germ agglutinin. Based on these results, we discuss the hypothesis that a selective CV-mediated pathway exists in developing skeletal muscle cells for the transport of CSQ from the early/intermediate Golgi apparatus to the SR.

Cell Physiology

Fundamental and applied biological sciences. Psychology

Biological and medical sciences

Molecular and cellular biology

Membrane and intracellular transports

Details

Title: Subtitle: Newly synthesized calsequestrin, destined for the sarcoplasmic reticulum, is contained in early/intermediate Golgi-derived clathrin-coated vesicles
Creators: Kathleen Thomas - Boston univ. school medicine, dep. microbiology, Boston MA 02118, United States
J NAVARRO - Boston univ. school medicine, dep. microbiology, Boston MA 02118, United States
Robin J. J Benson - Boston univ. school medicine, dep. microbiology, Boston MA 02118, United States
Kevin P Campbell - Boston univ. school medicine, dep. microbiology, Boston MA 02118, United States
R. L ROTUNDO - Boston univ. school medicine, dep. microbiology, Boston MA 02118, United States
R. E FINE - Boston univ. school medicine, dep. microbiology, Boston MA 02118, United States
Resource Type: Journal article
Publication Details: The Journal of biological chemistry, Vol.264(6), pp.3140-3145
DOI: 10.1016/S0021-9258(18)94042-1
PMID: 2563378
NLM abbreviation: J Biol Chem
ISSN: 0021-9258
eISSN: 1083-351X
Publisher: American Society for Biochemistry and Molecular Biology; Bethesda, MD
Language: English
Date published: 1989
Academic Unit: Neurology; Molecular Physiology and Biophysics; Iowa Neuroscience Institute
Record Identifier: 9984068263502771

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