Journal article
Next generation sequencing approaches to evaluate water and wastewater quality
Water research (Oxford), Vol.194, pp.116907-116907
04/15/2021
DOI: 10.1016/j.watres.2021.116907
PMID: 33610927
Abstract
The emergence of next generation sequencing (NGS) is revolutionizing the potential to address complex microbiological challenges in the water industry. NGS technologies can provide holistic insight into microbial communities and their functional capacities in water and wastewater systems, thus eliminating the need to develop a new assay for each target organism or gene. However, several barriers have hampered wide-scale adoption of NGS by the water industry, including cost, need for specialized expertise and equipment, challenges with data analysis and interpretation, lack of standardized methods, and the rapid pace of development of new technologies. In this critical review, we provide an overview of the current state of the science of NGS technologies as they apply to water, wastewater, and recycled water. In addition, a systematic literature review was conducted in which we identified over 600 peer-reviewed journal articles on this topic and summarized their contributions to six key areas relevant to the water and wastewater fields: taxonomic classification and pathogen detection, functional and catabolic gene characterization, antimicrobial resistance (AMR) profiling, bacterial toxicity characterization, Cyanobacteria and harmful algal bloom identification, and virus characterization. For each application, we have presented key trends, noteworthy advancements, and proposed future directions. Finally, key needs to advance NGS technologies for broader application in water and wastewater fields are assessed.
Details
- Title: Subtitle
- Next generation sequencing approaches to evaluate water and wastewater quality
- Creators
- Emily Garner - West Virginia UniversityBenjamin C Davis - Virginia TechErin Milligan - Virginia TechMatthew Forrest Blair - Virginia TechIshi Keenum - Virginia TechAyella Maile-Moskowitz - Virginia TechJin Pan - Virginia TechMariah Gnegy - Virginia TechKrista Liguori - Virginia TechSuraj Gupta - Virginia TechAaron J Prussin II - Virginia TechLinsey C Marr - Virginia TechLenwood S Heath - Virginia TechPeter J Vikesland - Virginia TechLiqing Zhang - Virginia TechAmy Pruden - Virginia Tech
- Resource Type
- Journal article
- Publication Details
- Water research (Oxford), Vol.194, pp.116907-116907
- DOI
- 10.1016/j.watres.2021.116907
- PMID
- 33610927
- NLM abbreviation
- Water Res
- ISSN
- 0043-1354
- eISSN
- 1879-2448
- Language
- English
- Date published
- 04/15/2021
- Academic Unit
- Occupational and Environmental Health
- Record Identifier
- 9984787258102771
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