Journal article
Ngly1, a mouse gene encoding a deglycosylating enzyme implicated in proteasomal degradation: expression, genomic organization, and chromosomal mapping
Biochemical and biophysical research communications, Vol.304(2), pp.326-332
2003
DOI: 10.1016/S0006-291X(03)00600-4
PMID: 12711318
Abstract
In species as diverse as yeast and mammals, peptide:
N-glycanase (
PNG1 in yeast;
Ngly1 in mouse) is believed to play a key role in the degradation of misfolded glycoproteins by the proteasome. In this study, we report the genomic organization and mRNA distribution of the mouse
Ngly1. Mouse
Ngly1 spans 61
kb and is composed of 12 exons, the organization of which is conserved throughout vertebrates. Comparison of the mouse and human genomic sequence identifies a conserved gene structure with significant sequence similarity extending into introns. A 2.6
kb
Ngly1 message was detected in all mouse tissues examined, with the highest abundance in the testis. In addition, a lower molecular weight transcript of 2.4
kb was detected in the testis. From analysis of dbESTs the alternative transcript of
Ngly1 is predicted to be present in the human placenta. Given the key role
Ngly1 plays in glycoprotein degradation, we predict that
Ngly1 may be a contributing factor in “disease” susceptibility. To begin to address this question, we used radiation hybrid mapping to localize mouse
Ngly1 to chromosome 14 and the human orthologue to chromosome 3 with a strong link with known genes.
Details
- Title: Subtitle
- Ngly1, a mouse gene encoding a deglycosylating enzyme implicated in proteasomal degradation: expression, genomic organization, and chromosomal mapping
- Creators
- Tadashi Suzuki - Stony Brook UniversityMichael A Kwofie - Stony Brook UniversityWilliam J Lennarz - Stony Brook University
- Resource Type
- Journal article
- Publication Details
- Biochemical and biophysical research communications, Vol.304(2), pp.326-332
- Publisher
- Elsevier Inc
- DOI
- 10.1016/S0006-291X(03)00600-4
- PMID
- 12711318
- ISSN
- 0006-291X
- eISSN
- 1090-2104
- Language
- English
- Date published
- 2003
- Academic Unit
- Radiology
- Record Identifier
- 9984318812002771
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