Nhe1 is essential for potassium but not calcium facilitation of cell motility and the monovalent cation requirement for chemotactic orientation in Dictyostelium discoideum

Daniel F Lusche; Deborah Wessels; Daniel E Ryerson; David R Soll

doi:10.1128/EC.00255-10

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Nhe1 is essential for potassium but not calcium facilitation of cell motility and the monovalent cation requirement for chemotactic orientation in Dictyostelium discoideum

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Nhe1 is essential for potassium but not calcium facilitation of cell motility and the monovalent cation requirement for chemotactic orientation in Dictyostelium discoideum

Daniel F Lusche, Deborah Wessels, Daniel E Ryerson and David R Soll

Eukaryotic cell, Vol.10(3), pp.320-331

03/2011

DOI: 10.1128/EC.00255-10

PMCID: PMC3067473

PMID: 21239624

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Abstract

In Dictyostelium discoideum, extracellular K+ or Ca2+ at a concentration of 40 or 20 mM, respectively, facilitates motility in the absence or presence of a spatial gradient of chemoattractant. Facilitation results in maximum velocity, cellular elongation, persistent translocation, suppression of lateral pseudopod formation, and myosin II localization in the posterior cortex. A lower threshold concentration of 15 mM K+ or Na or 5 mM Ca2+ is required for chemotactic orientation. Although the common buffer solutions used by D. discoideum researchers to study chemotaxis contain sufficient concentrations of cations for chemotactic orientation, the majority contain insufficient levels to facilitate motility. Here it has been demonstrated that Nhe1, a plasma membrane protein, is required for K+ but not Ca2+ facilitation of cell motility and for the lower K+ but not Ca2+ requirement for chemotactic orientation.

Potassium - metabolism

Protein Structure, Tertiary

Amino Acid Sequence

Cell Polarity

Calcium - metabolism

Membrane Proteins - genetics

Molecular Sequence Data

Protozoan Proteins - genetics

Chemotaxis

Protozoan Proteins - metabolism

Biological Transport

Membrane Proteins - chemistry

Cations, Monovalent - metabolism

Dictyostelium - physiology

Dictyostelium - chemistry

Dictyostelium - genetics

Protozoan Proteins - chemistry

Membrane Proteins - metabolism

Dictyostelium - cytology

Cell Movement

Details

Title: Subtitle: Nhe1 is essential for potassium but not calcium facilitation of cell motility and the monovalent cation requirement for chemotactic orientation in Dictyostelium discoideum
Creators: Daniel F Lusche - The W.M. Keck Dynamic Image Analysis Facility,Department of Biology, The University of Iowa, Iowa City, IA 52242, USA
Deborah Wessels
Daniel E Ryerson
David R Soll
Resource Type: Journal article
Publication Details: Eukaryotic cell, Vol.10(3), pp.320-331
DOI: 10.1128/EC.00255-10
PMID: 21239624
PMCID: PMC3067473
NLM abbreviation: Eukaryot Cell
ISSN: 1535-9778
eISSN: 1535-9786
Publisher: American Society for Microbiology; United States
Language: English
Date published: 03/2011
Academic Unit: Biology
Record Identifier: 9983991967702771

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