Journal article
Nicotine effects on PGE2 and IL-1β release by LPS-treated human monocytes
Journal of periodontal research, Vol.31(2), pp.99-104
1996
DOI: 10.1111/j.1600-0765.1996.tb00470.x
PMID: 8708946
Abstract
Cigarette smoking is a major risk factor in the development and further progression of periodontitis. However, little is known regarding the pathogenesis of smoking-related periodontal diseases. The purpose of this study was to examine the effects of nicotine, alone and in combination with lipopolysaccharide (LPS), on monocyte secretion of bone-resorbing factors, PGE2 and IL-1β. Peripheral blood monocytes (PBM) were isolated by counterflow centrifugal elutriation from 15 healthy, non-smoking donors. PBM were incubated for 24 h in RPMI 1640 containing nicotine (0, 50 μg/ml, I μg/ml, 10 μg/ml and 100 μg/ml) with or without 10 μg/ml Porphyromonas gingivalis LPS or Escherichia coli LPS. Culture supernatants were assayed for PGE2 and IL-1β by ELISA. None of the nicotine preparations resulted in significant PBM secretion of PGE2 and IL-1β above that of unstimulated cultures. However, PGE2 release was potentiated 1.7-fold by the combination of P. gingivalis LPS and 10 μg/ml nicotine relative to P. gingivalis LPS alone (p<0.05, one-way ANOVA). Prostaglandin E3 release also was potentiated 3.5-fold by P. gingivalis LPS and 100 μg/ml nicotine relative to P. gingivalis LPS alone (p<0.00001, one-way ANOVA) and 3.1-fold by E. coli LPS and 100 μg/ml nicotine relative to E. coli LPS alone (p<0.00001, I. one-way ANOVA). IL-1β secretion was lower for either LPS plus 100 μg/ml nicotine relative to LPS alone, although not significantly. These data demonstrate upregulation of LPS-mediated monocyte secretion of PGE2 by nicotine and suggest a potential role for nicotine in periodontal disease pathogenesis.
Details
- Title: Subtitle
- Nicotine effects on PGE2 and IL-1β release by LPS-treated human monocytes
- Creators
- J. B PAYNE - Immunoregulation Laboratories, Department of Surgical Specialties, University of Nebraska Medical Center, Lincoln, United StatesG. K JOHNSON - Immunoregulation Laboratories, Department of Surgical Specialties, University of Nebraska Medical Center, Lincoln, United StatesR. A REINHARDT - Immunoregulation Laboratories, Department of Surgical Specialties, University of Nebraska Medical Center, Lincoln, United StatesJ. K DYER - Immunoregulation Laboratories, Department of Surgical Specialties, University of Nebraska Medical Center, Lincoln, United StatesC. A MAZE - Immunoregulation Laboratories, Department of Surgical Specialties, University of Nebraska Medical Center, Lincoln, United StatesD. G DUNNING - Immunoregulation Laboratories, Department of Surgical Specialties, University of Nebraska Medical Center, Lincoln, United States
- Resource Type
- Journal article
- Publication Details
- Journal of periodontal research, Vol.31(2), pp.99-104
- Publisher
- Blackwell; Oxford
- DOI
- 10.1111/j.1600-0765.1996.tb00470.x
- PMID
- 8708946
- ISSN
- 0022-3484
- eISSN
- 1600-0765
- Language
- English
- Date published
- 1996
- Academic Unit
- Periodontics
- Record Identifier
- 9984065990702771
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