Journal article
Nitric Oxide Inhibits Iron–Induced Lipid Peroxidation in HL-60 Cells
Archives of biochemistry and biophysics, Vol.370(1), pp.97-104
10/01/1999
DOI: 10.1006/abbi.1999.1386
PMID: 10496982
Abstract
Nitric oxide (•NO) can protect cells against the detrimental effects of reactive oxygen species. Using low-density lipoprotein as well as model systems, it has been demonstrated that •NO can serve as a chain-breaking antioxidant to blunt lipid peroxidation. To test the hypothesis that •NO can serve as a chain-breaking antioxidant in cell membranes, we examined the effect of •NO on iron-induced lipid peroxidation in human leukemia cells. We exposed HL-60 cells to an oxidative stress (20 μM Fe2+) and monitored the consumption of oxygen as a measure of lipid peroxidation. Oxygen consumption was arrested by the addition of •NO as a saturated aqueous solution. The duration of inhibition of oxygen consumption by •NO was concentration-dependent in the 0.4–1.8 μM range. The inhibition ended upon depletion of •NO. The addition of •NO prior to initiation of peroxidation delayed the onset of peroxidation; the nearer in time it was before Fe2+ addition, the longer the inhibition. Depletion of cellular glutathione levels by d,l-buthionine-S,R-sulfoximine prior to Fe2+ addition resulted in a more rapid initial rate of oxygen depletion and a shorter time for the •NO-induced inhibition of oxygen consumption. Complementary studies of this iron-induced lipid peroxidation, using thiobarbituric acid reactive substances as a marker, also demonstrated the protective effects of •NO. This protection of cells against lipid peroxidation also manifested itself as a reduction in trypan blue uptake, an observation demonstrating the protective effects of •NO on membrane integrity. We conclude that •NO protects HL-60 human leukemia cells from lipid peroxidation and that this protection ameliorates the toxicity of the oxidation processes initiated by Fe2+ and dioxygen.
Details
- Title: Subtitle
- Nitric Oxide Inhibits Iron–Induced Lipid Peroxidation in HL-60 Cells
- Creators
- Eric E Kelley - Department of Medicine, The University of Iowa College of Medicine, The University of Iowa Cancer Center, Iowa City, Iowa, 52242Brett A Wagner - Department of Medicine, The University of Iowa College of Medicine, The University of Iowa Cancer Center, Iowa City, Iowa, 52242Garry R Buettner - Department of Radiology, The University of Iowa College of Medicine, The University of Iowa Cancer Center, Iowa City, Iowa, 52242C.Patrick Burns - Department of Medicine, The University of Iowa College of Medicine, The University of Iowa Cancer Center, Iowa City, Iowa, 52242
- Resource Type
- Journal article
- Publication Details
- Archives of biochemistry and biophysics, Vol.370(1), pp.97-104
- Publisher
- Elsevier Inc
- DOI
- 10.1006/abbi.1999.1386
- PMID
- 10496982
- ISSN
- 0003-9861
- eISSN
- 1096-0384
- Language
- English
- Date published
- 10/01/1999
- Academic Unit
- Hematology, Oncology, and Blood & Marrow Transplantation; Radiation Oncology; Internal Medicine
- Record Identifier
- 9984047980602771
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