Journal article
Nitric oxide and S-nitroso-L-cysteine as endothelium-derived relaxing factors from acetylcholine in cerebral vessels in cats
Stroke (1970), Vol.24(12), pp.2010-2014
12/1993
DOI: 10.1161/01.STR.24.12.2010
PMID: 8248984
Abstract
Background and purpose: The predominant view is that the endothelium-derived relaxing factor generated by acetylcholine from blood vessels is nitric oxide. However, there is evidence suggesting that certain nitric oxide-containing compounds such as nitrosothiols resemble the endothelium-derived relaxing factor generated by acetylcholine more closely than does nitric oxide itself. Accordingly, we compared the effects of nitric oxide and S-nitroso-L-cysteine on cerebral arteriolar caliber in relation to the associated increments in nitrite concentration in the effluent.
Methods: Acetylcholine, nitric oxide, and S-nitroso-L-cysteine were administered by continuous superfusion in oxygen-free solution through the space under a cranial window in anesthetized cats. Nitrite concentration was measured in the effluent. The degree of vasodilation induced was evaluated in relation to the increment in nitrite concentration.
Results: All agents induced dose-dependent vasodilation and dose-dependent increments in nitrite concentration in the effluent. For any given degree of vasodilation, the increments in nitrite concentration were equivalent during acetylcholine or S-nitroso-L-cysteine infusion, whereas the nitrite concentrations were 10 times higher during nitric oxide infusion. After administration of nitroarginine, a competitive inhibitor of nitric oxide synthesis from arginine, there was depression in the vasodilation as well as the increment in nitrite concentration induced by acetylcholine.
Conclusions: S-Nitroso-L-cysteine resembles endothelium-derived relaxing factor from acetylcholine more closely than does nitric oxide.
Details
- Title: Subtitle
- Nitric oxide and S-nitroso-L-cysteine as endothelium-derived relaxing factors from acetylcholine in cerebral vessels in cats
- Creators
- R C Kukreja - Department of Medicine, Medical College of Virginia, Richmond 23298E P Wei - Department of Medicine, Medical College of Virginia, Richmond 23298H A Kontos - Department of Medicine, Medical College of Virginia, Richmond 23298J N Bates - Department of Medicine, Medical College of Virginia, Richmond 23298
- Resource Type
- Journal article
- Publication Details
- Stroke (1970), Vol.24(12), pp.2010-2014
- DOI
- 10.1161/01.STR.24.12.2010
- PMID
- 8248984
- NLM abbreviation
- Stroke
- ISSN
- 0039-2499
- eISSN
- 1524-4628
- Language
- English
- Date published
- 12/1993
- Academic Unit
- Anesthesia
- Record Identifier
- 9984006442202771
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