Journal article
Nitric oxide generation from nitroprusside by vascular tissue: Evidence that reduction of the nitroprusside anion and cyanide loss are required
Biochemical pharmacology, Vol.42(1), pp.S157-S165
1991
DOI: 10.1016/0006-2952(91)90406-U
PMID: 1768273
Abstract
Nitric oxide (NO) was produced from sodium nitroprusside in the presence of vascular tissue but was not released spontaneously from the nitroprusside anion. In the absence of tissue in the dark nitroprusside did not release NO. When solutions of nitroprusside alone were irradiated with visible light, nitric oxide was released at rates linearly proportional to nitroprusside concentration and light intensity. Nitric oxide was produced from solutions of nitroprusside in the dark after the addition of vascular tissue, including lengths of rabbit aorta, subcellular fractions of aorta, and human plasma. NO was also released from nitroprusside after reaction with various reducing agents including cysteine and other thiols, ascorbic acid, sodium dithionite, ferrous chloride, hemoglobin, myoglobin, and partially purified cytochrome P450 with an NADPH-regenerating system. HCN was simultaneously produced in these solutions, and addition of KCN blocked NO release. Iodine oxidized intermediate cyanoferrates and blocked nitric oxide release. KCN or iodine also blocked NO production by tissue, but had no effect upon photochemical NO release. These results show that, apart from photolysis which makes no physiological contribution, release of nitric oxide from nitroprusside, in simple solutions and in biological tissue, occurs after nitroprusside has undergone reduction and lost cyanide.
Details
- Title: Subtitle
- Nitric oxide generation from nitroprusside by vascular tissue: Evidence that reduction of the nitroprusside anion and cyanide loss are required
- Creators
- James N Bates - The Cardiovascular Institute and Departments of Anesthesia University of Iowa College of Medicine, Iowa City, IA 52242, U.S.AMax T Baker - The Cardiovascular Institute and Departments of Anesthesia University of Iowa College of Medicine, Iowa City, IA 52242, U.S.ARicardo Guerra - The Cardiovascular Institute and Departments ofInternal Medicine University of Iowa College of Medicine, Iowa City, IA 52242, U.S.ADavid G Harrison - The Cardiovascular Institute and Departments ofInternal Medicine University of Iowa College of Medicine, Iowa City, IA 52242, U.S.A
- Resource Type
- Journal article
- Publication Details
- Biochemical pharmacology, Vol.42(1), pp.S157-S165
- Publisher
- Elsevier Inc
- DOI
- 10.1016/0006-2952(91)90406-U
- PMID
- 1768273
- ISSN
- 0006-2952
- eISSN
- 1873-2968
- Language
- English
- Date published
- 1991
- Academic Unit
- Anesthesia
- Record Identifier
- 9984007188102771
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