Nitric oxide: mediator of NANC hyperpolarization of opossum esophageal smooth muscle

C Du; J Murray; J N Bates; J L Conklin

doi:10.1152/ajpgi.1991.261.6.G1012

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Nitric oxide: mediator of NANC hyperpolarization of opossum esophageal smooth muscle

Journal article

Peer reviewed

Nitric oxide: mediator of NANC hyperpolarization of opossum esophageal smooth muscle

C Du, J Murray, J N Bates and J L Conklin

The American journal of physiology, Vol.261(6 Pt 1), pp.G1012-G1016

12/1991

DOI: 10.1152/ajpgi.1991.261.6.G1012

PMID: 1722645

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Abstract

Activation of intrinsic nonadrenergic noncholinergic (NANC) esophageal nerves during peristalsis or by electrical field stimulation (EFS) in vitro produces a hyperpolarization followed by a depolarization of the circular smooth muscle of the opossum esophagus. N omega-nitro-L-arginine (L-NNA), an inhibitor of nitric oxide synthase, and nitric oxide (NO) were used to test the hypothesis that NO or a NO-containing compound is a mediator of this NANC nerve-induced hyperpolarization of circular esophageal smooth muscle. The transmembrane potential difference of esophageal circular smooth muscle cells was recorded with glass microelectrodes. Nerve-mediated membrane responses were evoked by single electrical pulses of 0.5 ms duration and 50 V amplitude. L-NNA abolished the initial hyperpolarization and reduced the amplitude of and the time to maximal depolarization. L-Arginine (1 mM), the substrate for NO synthase, antagonized the effect of L-NNA. Exogenous NO produced hyperpolarization of the smooth muscle membrane potential and attenuated the amplitudes of EFS-induced hyperpolarization and depolarization. The effect of NO was blocked neither by L-NNA nor by tetrodotoxin (1 microM). The data support the hypothesis that NO or a NO-containing compound mediates NANC nerve-induced responses of the esophageal smooth muscle membrane.

Esophagus - drug effects

Electric Stimulation

Muscle, Smooth - innervation

Opossums

Amino Acid Oxidoreductases - antagonists & inhibitors

Male

Esophagus - physiology

Amino Acid Oxidoreductases - metabolism

Arginine - analogs & derivatives

Muscle, Smooth - drug effects

Animals

Membrane Potentials

Arginine - pharmacology

Nitric Oxide Synthase

Female

Muscle, Smooth - physiology

Nitroarginine

Esophagus - innervation

Nitric Oxide - metabolism

Details

Title: Subtitle: Nitric oxide: mediator of NANC hyperpolarization of opossum esophageal smooth muscle
Creators: C Du - Department of Internal Medicine, University of Iowa College of Medicine, Iowa City
J Murray
J N Bates
J L Conklin
Resource Type: Journal article
Publication Details: The American journal of physiology, Vol.261(6 Pt 1), pp.G1012-G1016
DOI: 10.1152/ajpgi.1991.261.6.G1012
PMID: 1722645
NLM abbreviation: Am J Physiol
ISSN: 0002-9513
eISSN: 2163-5773
Publisher: American Physiological Society; United States
Grant note: DK-01750 / NIDDK NIH HHS
Language: English
Date published: 12/1991
Academic Unit: Anesthesia
Record Identifier: 9984006429302771

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