Nitric oxide reverses prednisolone-induced inactivation of muscle satellite cells

Jenna L Betters; Jodi H D Long; Kathleen S Howe; Randy W Braith; Quinlyn A Soltow; Vitor A Lira; David S Criswell

doi:10.1002/mus.20915

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Nitric oxide reverses prednisolone-induced inactivation of muscle satellite cells

Journal article

Peer reviewed

Nitric oxide reverses prednisolone-induced inactivation of muscle satellite cells

Jenna L Betters, Jodi H D Long, Kathleen S Howe, Randy W Braith, Quinlyn A Soltow, Vitor A Lira and David S Criswell

Muscle & nerve, Vol.37(2), pp.203-209

02/2008

DOI: 10.1002/mus.20915

PMID: 18004769

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Abstract

Long-term corticosteroid therapy causes myopathy and can inhibit regeneration of skeletal muscle. Therefore, we hypothesized that corticosteroid exposure reduces satellite cell activity in skeletal myofibers. Male Swiss-Webster mice were injected daily for 8 weeks with prednisolone (GC) or vehicle (control). Single myofibers were isolated from the gastrocnemius, centrifuged to mechanically activate satellite cells, and maintained in culture for 48 h. Both constitutive nitric oxide synthase (NOS) isoforms were reduced in muscle by GC treatment (nNOS: -30%, eNOS: -34%). Fewer myogenic (myoD+) cells emanated from GC myofibers compared to control (-61%, P < 0.05). Supplementation of culture media with the nitric oxide donor, diethylenetriamine NONOate (DETA-NO; 5-50 microM), caused a dose-dependent increase in the number of myoD+ cells arising from both control and GC myofibers (P < 0.05), and 10 and 50 microM DETA-NO eliminated the GC-induced deficit in myogenic cells (P > 0.05). Therefore, supplementation of GC myofibers with DETA-NO restores satellite cell activity to control levels. Nitric oxide production could be an important therapeutic target for the prevention of corticosteroid myopathy.

Cells, Cultured

Male

MyoD Protein - metabolism

Prednisolone - pharmacology

Dose-Response Relationship, Drug

Satellite Cells, Skeletal Muscle - drug effects

Animals

Drug Interactions

Protein Isoforms - metabolism

Analysis of Variance

Glucocorticoids - pharmacology

Triazenes - pharmacology

Mice

Nitric Oxide Synthase - metabolism

Nitric Oxide Donors - pharmacology

Details

Title: Subtitle: Nitric oxide reverses prednisolone-induced inactivation of muscle satellite cells
Creators: Jenna L Betters - Center for Exercise Science, Department of Applied Physiology & Kinesiology, University of Florida, Gainesville, Florida 32611, USA
Jodi H D Long
Kathleen S Howe
Randy W Braith
Quinlyn A Soltow
Vitor A Lira
David S Criswell
Resource Type: Journal article
Publication Details: Muscle & nerve, Vol.37(2), pp.203-209
Publisher: United States
DOI: 10.1002/mus.20915
PMID: 18004769
ISSN: 0148-639X
eISSN: 1097-4598
Grant note: DOI: 10.13039/100000968, name: American Heart Association
Language: English
Date published: 02/2008
Academic Unit: Health and Human Physiology
Record Identifier: 9984002373502771

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