Journal article
Nitric oxide stimulates insulin release in liver cells expressing human insulin
Biochemical and biophysical research communications, Vol.329(4), pp.1329-1333
04/22/2005
DOI: 10.1016/j.bbrc.2005.02.111
PMID: 15766572
Abstract
The establishment of surrogate islet β cells is important for the treatment of diabetes. Hepatocytes have a similar glucose sensing system as β cells and have the potential to serve as surrogate β cells. In this report, we demonstrate that infection of Hepa1–6 liver cells with a lentivirus expressing the human insulin cDNA results in expression and secretion of human insulin. Furthermore, we show that
l-arginine at low levels of glucose significantly stimulates the release of insulin from these cells, compared to exposure to high concentration of glucose. The arginine-induced insulin release is via the production of nitric oxide, since treatment with
N
G-nitro-
l-arginine, an inhibitor of nitric oxide synthase, blocks insulin secretion induced by
l-arginine. These results indicate that nitric oxide plays a role in
l-arginine-stimulated insulin release in hepatocytes expressing the human insulin gene, and provides a new strategy to induce insulin secretion from engineered non-β cells.
Details
- Title: Subtitle
- Nitric oxide stimulates insulin release in liver cells expressing human insulin
- Creators
- Qingwen Qian - University of KentuckyJohn P. Williams - University of KentuckyDennis G. Karounos - University of KentuckySabire Özcan - University of Kentucky
- Resource Type
- Journal article
- Publication Details
- Biochemical and biophysical research communications, Vol.329(4), pp.1329-1333
- Publisher
- Elsevier Inc
- DOI
- 10.1016/j.bbrc.2005.02.111
- PMID
- 15766572
- ISSN
- 0006-291X
- eISSN
- 1090-2104
- Language
- English
- Date published
- 04/22/2005
- Academic Unit
- Anatomy and Cell Biology
- Record Identifier
- 9984420935802771
Metrics
2 Record Views