Nitric oxide synthase Type II expression by different cell types in MHV-JHM encephalitis suggests distinct roles for nitric oxide in acute versus persistent virus infection

Dana M Grzybicki; KyuBum Kwack; Stanley Perlman; Sean P Murphy

doi:10.1016/S0165-5728(96)00159-2

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Nitric oxide synthase Type II expression by different cell types in MHV-JHM encephalitis suggests distinct roles for nitric oxide in acute versus persistent virus infection

Journal article

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Nitric oxide synthase Type II expression by different cell types in MHV-JHM encephalitis suggests distinct roles for nitric oxide in acute versus persistent virus infection

Dana M Grzybicki, KyuBum Kwack, Stanley Perlman and Sean P Murphy

Journal of Neuroimmunology, Vol.73(1), pp.15-27

03/1997

DOI: 10.1016/S0165-5728(96)00159-2

PMCID: PMC7119606

PMID: 9058755

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Abstract

Intranasal inoculation with mouse hepatitis virus strain JHM (MHV-JHM) results in acute meningoencephalitis. We found NOS II mRNA expression in brains of acutely infected animals on days 5 through 7 after infection. In situ hybridization and immunohistochemistry demonstrated NOS II message and protein in infiltrating macrophages. Persistent infection with MHV-JHM results in chronic demyelinating encephalomyelitis. NOS II mRNA was detected in persistently infected spinal cords. In situ hybridization and immunohistochemistry showed expression of NOS II in astrocytes in and around demyelinated lesions. These results suggest the role of NO release in acute versus persistent infection with this virus, and its contribution to the resulting pathology, may be different.

Immunohistochemistry

Macrophage

Virus

Astrocyte

Demyelination

Nitric oxide

Details

Title: Subtitle: Nitric oxide synthase Type II expression by different cell types in MHV-JHM encephalitis suggests distinct roles for nitric oxide in acute versus persistent virus infection
Creators: Dana M Grzybicki - Department of Pathology, 106 ML, University of Iowa College of Medicine, Iowa City, IA 52242, USA
KyuBum Kwack - Department of Pathology, 106 ML, University of Iowa College of Medicine, Iowa City, IA 52242, USA
Stanley Perlman - Department of Pediatrics, University of Iowa College of Medicine, Iowa City, IA 52242, USA
Sean P Murphy - Department of Pharmacology, University of Iowa College of Medicine, Iowa City, IA 52242, USA
Resource Type: Journal article
Publication Details: Journal of Neuroimmunology, Vol.73(1), pp.15-27
DOI: 10.1016/S0165-5728(96)00159-2
PMID: 9058755
PMCID: PMC7119606
NLM abbreviation: J Neuroimmunol
ISSN: 0165-5728
eISSN: 1872-8421
Publisher: Elsevier B.V
Language: English
Date published: 03/1997
Academic Unit: Microbiology and Immunology; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Infectious Disease (Pediatrics)
Record Identifier: 9983777474902771

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