Nitrite Reductase NirS Is Required for Type III Secretion System Expression and Virulence in the Human Monocyte Cell Line THP-1 by Pseudomonas aeruginosa

Nadine E Van Alst; Melanie Wellington; Virginia L Clark; Constantine G Haidaris; Barbara H Iglewski

doi:10.1128/IAI.00822-09

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Nitrite Reductase NirS Is Required for Type III Secretion System Expression and Virulence in the Human Monocyte Cell Line THP-1 by Pseudomonas aeruginosa

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Nitrite Reductase NirS Is Required for Type III Secretion System Expression and Virulence in the Human Monocyte Cell Line THP-1 by Pseudomonas aeruginosa

Nadine E Van Alst, Melanie Wellington, Virginia L Clark, Constantine G Haidaris and Barbara H Iglewski

Infection and immunity, Vol.77(10), pp.4446-4454

10/2009

DOI: 10.1128/IAI.00822-09

PMCID: PMC2747934

PMID: 19651860

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Abstract

The nitrate dissimilation pathway is important for anaerobic growth in Pseudomonas aeruginosa . In addition, this pathway contributes to P. aeruginosa virulence by using the nematode Caenorhabditis elegans as a model host, as well as biofilm formation and motility. We used a set of nitrate dissimilation pathway mutants to evaluate the virulence of P. aeruginosa PA14 in a model of P. aeruginosa -phagocyte interaction by using the human monocytic cell line THP-1. Both membrane nitrate reductase and nitrite reductase enzyme complexes were important for cytotoxicity during the interaction of P. aeruginosa PA14 with THP-1 cells. Furthermore, deletion mutations in genes encoding membrane nitrate reductase (Δ narGH ) and nitrite reductase (Δ nirS ) produced defects in the expression of type III secretion system (T3SS) components, extracellular protease, and elastase. Interestingly, exotoxin A expression was unaffected in these mutants. Addition of exogenous nitric oxide (NO)-generating compounds to Δ nirS mutant cultures restored the production of T3SS phospholipase ExoU, whereas nitrite addition had no effect. These data suggest that NO generated via nitrite reductase NirS contributes to the regulation of expression of selected virulence factors in P. aeruginosa PA14.

Molecular Pathogenesis

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Title: Subtitle: Nitrite Reductase NirS Is Required for Type III Secretion System Expression and Virulence in the Human Monocyte Cell Line THP-1 by Pseudomonas aeruginosa
Creators: Nadine E Van Alst - Department of Microbiology and Immunology
Melanie Wellington - Department of Microbiology and Immunology
Virginia L Clark - Department of Microbiology and Immunology
Constantine G Haidaris - Department of Microbiology and Immunology
Barbara H Iglewski - Department of Microbiology and Immunology
Resource Type: Journal article
Publication Details: Infection and immunity, Vol.77(10), pp.4446-4454
DOI: 10.1128/IAI.00822-09
PMID: 19651860
PMCID: PMC2747934
NLM abbreviation: Infect Immun
ISSN: 0019-9567
eISSN: 1098-5522
Publisher: American Society for Microbiology (ASM)
Language: English
Date published: 10/2009
Academic Unit: Stead Family Department of Pediatrics; Infectious Disease (Pediatrics)
Record Identifier: 9984093345402771

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