Journal article
Non-cytotoxic concentrations of acetaminophen induced mitochondrial biogenesis and antioxidant response in HepG2 cells
Environmental toxicology and pharmacology, Vol.46, pp.71-79
09/2016
DOI: 10.1016/j.etap.2016.06.030
PMID: 27438896
Abstract
•Non-cytotoxic concentration of APAP induced mitochondrial biogenesis in HepG2 cells.•The heightened biogenesis concurred with induction of PGC-1α, NRF-1 and TFAM genes.•GSH and antioxidant genes were also induced by non-cytotoxic concentration of APAP.•The above two groups of genes were downregulated at cytotoxic concentration of APAP.•Mitochondrial biogenesis and antioxidant response could protect liver from APAP.
Mitochondrial dysfunction has been implicated in acute, severe liver injury caused by overdose of acetaminophen (APAP). However, whether mitochondrial biogenesis is involved is unclear. Here we demonstrated that mitochondrial biogenesis, as indicated by the amounts of mitochondrial DNA and proteins, increased significantly in HepG2 cells exposed to low, non-cytotoxic concentrations of APAP. This heightened response was accompanied by upregulated expression of PGC-1α, NRF-1 and TFAM, which are key transcriptional regulators of mitochondrial biogenesis. Additionally, antioxidants including glutathione, MnSOD, HO-1, NQO1, and Nrf2 were also significantly upregulated. In contrast, for HepG2 cells exposed to high, cytotoxic concentration of APAP, mitochondrial biogenesis was inhibited and the expression of its regulatory proteins and antioxidants were concentration-dependently downregulated. In summary, our study indicated that mitochondrial biogenesis, along with antioxidant induction, may be an important cellular adaptive mechanism counteracting APAP-induced toxicity and overwhelming this cytoprotective capacity could result in liver injury.
Details
- Title: Subtitle
- Non-cytotoxic concentrations of acetaminophen induced mitochondrial biogenesis and antioxidant response in HepG2 cells
- Creators
- Tingfen Zhang - Academy of Military Medical SciencesQiang Zhang - Emory UniversityJiabin Guo - Academy of Military Medical SciencesHaitao Yuan - Academy of Military Medical SciencesHui Peng - Academy of Military Medical SciencesLan Cui - Academy of Military Medical SciencesJian Yin - Academy of Military Medical SciencesLi Zhang - Academy of Military Medical SciencesJun Zhao - Academy of Military Medical SciencesJin LiAndrew White - UnileverPaul L. Carmichael - UnileverCarl Westmoreland - UnileverShuangqing Peng - Academy of Military Medical Sciences
- Resource Type
- Journal article
- Publication Details
- Environmental toxicology and pharmacology, Vol.46, pp.71-79
- Publisher
- Elsevier B.V
- DOI
- 10.1016/j.etap.2016.06.030
- PMID
- 27438896
- ISSN
- 1382-6689
- eISSN
- 1872-7077
- Grant note
- DOI: 10.13039/501100001809, name: National Natural Science Foundation of China, award: 81302864, 81430090; name: Unilever International Collaborative Support, award: 2013-00202
- Language
- English
- Date published
- 09/2016
- Academic Unit
- Neurology
- Record Identifier
- 9984303019902771
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