Journal article
Non-invasive electromyographic estimation of motor unit number in the external anal sphincter of the rat
Neurourology and urodynamics, Vol.37(1), pp.115-122
01/2018
DOI: 10.1002/nau.23313
PMID: 28884835
Abstract
AimsThe external anal sphincter (EAS) is essential for maintaining fecal continence. Neurological disorders or traumatic injuries to muscle and nervous systems could lead to EAS denervation. Currently, there are no techniques available to document global innervation changes in the EAS in vivo. The aim of this study was to develop a novel approach to non-invasively estimate the number of functioning motor units (MUs) in the EAS and validate with immunofluorescent techniques in rats.
MethodsIntra-rectal surface electromyography (EMG) signals of the EAS, induced by a series of intra-vaginally delivered pudendal nerve stimulations with different intensities, were recorded. Variation in EMG responses at different intensities was used to estimate the value of a single motor unit potential (SMUP) in order to perform the proposed EAS motor unit number estimation (MUNE) approach. The EAS MUNE was tested in 12 female Sprague-Dawley rats, and validated by comparing against the EAS myofiber counting results achieved by performing immunostaining of acetylcholine receptors in 7 of the 12 rats.
ResultsThe mean MU number was 359, with an averaged SMUP size of 52.49 +/- 20.39V. The mean number of successfully identified myofibers was 652.7 +/- 130.6 myofiber/EAS. Significance of linear regression between the immunofluorescent results and the MUNE was confirmed (P<0.01).
ConclusionsOur study represents the first effort to non-invasively assess the innervation of the EAS in vivo using the rat as a pre-clinical model. This approach can potentially enable future clinical applications for advanced diagnosis and treatment of neurogenic EAS disorders.
Details
- Title: Subtitle
- Non-invasive electromyographic estimation of motor unit number in the external anal sphincter of the rat
- Creators
- Chuan Zhang - University of HoustonAlvaro Munoz - Houston MethodistH. Henry Lai - Washington University in St. LouisTimothy Boone - Houston MethodistYingchun Zhang - Houston Methodist
- Resource Type
- Journal article
- Publication Details
- Neurourology and urodynamics, Vol.37(1), pp.115-122
- DOI
- 10.1002/nau.23313
- PMID
- 28884835
- NLM abbreviation
- Neurourol Urodyn
- ISSN
- 0733-2467
- eISSN
- 1520-6777
- Publisher
- Wiley
- Number of pages
- 8
- Grant note
- Houston Methodist foundation University of Houston DK082644 / National Institute of Diabetes and Digestive and Kidney Diseases; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Diabetes & Digestive & Kidney Diseases (NIDDK) Brown Foundation K99DK082644 / NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Diabetes & Digestive & Kidney Diseases (NIDDK)
- Language
- English
- Date published
- 01/2018
- Academic Unit
- Urology
- Record Identifier
- 9985244653102771
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