Journal article
Non-isotopic polymerase chain reaction methods for the detection of HIV-1 in Ugandan mothers and infants
AIDS (London), Vol.5(12), pp.1463-1467
12/1991
DOI: 10.1097/00002030-199112000-00008
PMID: 1814329
Abstract
Two non-isotopic polymerase chain reaction (PCR) methods were evaluated by testing blood from 41 HIV-1-seropositive and 16 HIV-1-seronegative Ugandan mothers and 56 of their children (aged 0.5-15.0 months). Amplification of HIV-1 sequences was performed in duplicate using a biotinylated primer pair to the gag region (SK 462-431) and nested primer pairs (JA 17-20) to the pol region of HIV-1. gag sequences were hybridized using a microtiter plate coated with the SK 102 probe followed by colorimetric detection using an avidin-horseradish peroxidase conjugate and tetramethylbenzidine/peroxide substrate. pol sequences were detected on agarose gel stained with ethidium bromide. Results of HIV-1 PCR analysis showed that 40 out of 41 (98%) seropositive mothers and 10 out of 29 (34%) seropositive children had detectable HIV-1 gag and pol sequences. None of the 16 seronegative mothers nor 27 seronegative or Western blot-indeterminate children had detectable HIV-1 sequences. Our results suggest that non-isotopic PCR methods are sensitive, specific, and potentially useful in the early diagnosis of HIV-1 infection in developed and developing countries.
Details
- Title: Subtitle
- Non-isotopic polymerase chain reaction methods for the detection of HIV-1 in Ugandan mothers and infants
- Creators
- J Brooks JacksonChristopher NdugwaFrancis MmiroPeter KataahaLaura GuayElizabeth A DragonJohanna GoldfarbKaren Olness
- Resource Type
- Journal article
- Publication Details
- AIDS (London), Vol.5(12), pp.1463-1467
- DOI
- 10.1097/00002030-199112000-00008
- PMID
- 1814329
- ISSN
- 0269-9370
- eISSN
- 1473-5571
- Language
- English
- Date published
- 12/1991
- Academic Unit
- Pathology; VPMA - Administration
- Record Identifier
- 9984047608402771
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