Journal article
Non-typeable Haemophilus influenzae protects human airway epithelial cells from a subsequent respiratory syncytial virus challenge
Virology (New York, N.Y.), Vol.498, pp.128-135
11/2016
DOI: 10.1016/j.virol.2016.08.020
PMCID: PMC5017537
PMID: 27573069
Abstract
Respiratory syncytial virus (RSV) and the common commensal and opportunistic pathogen, non-typeable Haemophilus influenzae (NTHi) both serve as a frequent cause of respiratory infection in children. Although it is well established that some respiratory viruses can increase host susceptibility to secondary bacterial infections, few studies have examined how commensal bacteria could influence a secondary viral response. Here, we examined the impact of NTHi exposure on a subsequent RSV infection of human bronchial epithelial cells (16HBE14o-). Co-culture of 16HBE14o- cells with NTHi 2019 resulted in inhibition of viral gene expression following RSV infection. 16HBE14o- cells co-cultured with heat-killed NTHi 2019 failed to protect against an RSV infection, indicating that protection requires live bacteria. However, NTHi did not inhibit influenza A virus replication, indicating that NTHi-mediated protection was RSV-specific. Our data demonstrates that prior exposure to a commensal bacterium such as NTHi can elicit protection against a subsequent RSV infection.
Details
- Title: Subtitle
- Non-typeable Haemophilus influenzae protects human airway epithelial cells from a subsequent respiratory syncytial virus challenge
- Creators
- Stacey M Hartwig - Department of Microbiology, University of Iowa, Iowa City, Iowa, United States of AmericaMargaret Ketterer - Department of Microbiology, University of Iowa, Iowa City, Iowa, United States of AmericaMichael A Apicella - Department of Microbiology, University of Iowa, Iowa City, Iowa, United States of AmericaSteven M Varga - Department of Microbiology, University of Iowa, Iowa City, Iowa, United States of America
- Resource Type
- Journal article
- Publication Details
- Virology (New York, N.Y.), Vol.498, pp.128-135
- DOI
- 10.1016/j.virol.2016.08.020
- PMID
- 27573069
- PMCID
- PMC5017537
- NLM abbreviation
- Virology
- ISSN
- 0042-6822
- eISSN
- 1096-0341
- Grant note
- name: Vice President for Research & Economic Development; DOI: 10.13039/100011343, name: Holden Comprehensive Cancer Center, award: P30 CA086862; DOI: 10.13039/100015515, name: Carver College of Medicine, award: 1 S10 RR025439-01
- Language
- English
- Date published
- 11/2016
- Academic Unit
- Graduate College Admin and Gen; Microbiology and Immunology; Pathology; Internal Medicine
- Record Identifier
- 9984083272402771
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