Journal article
Nondestructive evaluation of osteogenic differentiation in tissue‐engineered constructs
Journal of orthopaedic research, Vol.24(5), pp.889-897
05/2006
DOI: 10.1002/jor.20140
PMID: 16583444
Abstract
Conventional measurements of osteogenesis in tissue‐engineered constructs are destructive to living cells and incapable to provide three‐dimensional information. In the present study, noninvasive magnetic resonance (MR) microscopy was used to evaluate osteogenic differentiation in vitro in human mesenchymal stem cell‐based tissue‐engineered constructs. The constructs were prepared by seeding the cells (106cells/ml) on 4 × 4 × 4 mm gelatin sponge cubes and subsequently exposing them to osteogenic differentiation or basic medium. During the 4‐week experiment, alkaline phosphatase (ALP) activity and calcium content of differentiated constructs were significantly increased compared to the basic medium controls. The T1, T2, and apparent diffusion coefficient (ADC) of differentiated constructs were significantly lower than those of the control group at each time point (p < 0.05). The MR parameters of constructs were significantly correlated to their ALP activities (r to T1, T2, and ADC = −0.57, −0.78, and −0.81, respectively) and calcium content (r to T1, T2, and ADC = 0.48, 0.90, and 0.92, respectively) measured by biochemical techniques. MR microscopy can be a promising tool for noninvasive assessment of osteogenic differentiation and to provide three‐dimensional information about tissue‐engineered constructs. © 2006 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res
Details
- Title: Subtitle
- Nondestructive evaluation of osteogenic differentiation in tissue‐engineered constructs
- Creators
- Liu HongIoana A PeptanHuihui XuRichard L Magin
- Resource Type
- Journal article
- Publication Details
- Journal of orthopaedic research, Vol.24(5), pp.889-897
- DOI
- 10.1002/jor.20140
- PMID
- 16583444
- NLM abbreviation
- J Orthop Res
- ISSN
- 0736-0266
- eISSN
- 1554-527X
- Publisher
- Wiley Subscription Services, Inc., A Wiley Company; Hoboken
- Number of pages
- 9
- Language
- English
- Date published
- 05/2006
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Prosthodontics; Craniofacial Anomalies Research Center; Dental Research
- Record Identifier
- 9984066095302771
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