Non‐invasive Salmonella typhimurium mutants are avirulent because of an inability to enter and destroy M cells of ileal Peyer's patches

Kristi L Penheiter; Nitin Mathur; Dobie Giles; Thomas Fahlen; Bradley D Jones

doi:10.1046/j.1365-2958.1997.3741745.x

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Non‐invasive Salmonella typhimurium mutants are avirulent because of an inability to enter and destroy M cells of ileal Peyer's patches

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Non‐invasive Salmonella typhimurium mutants are avirulent because of an inability to enter and destroy M cells of ileal Peyer's patches

Kristi L Penheiter, Nitin Mathur, Dobie Giles, Thomas Fahlen and Bradley D Jones

Molecular microbiology, Vol.24(4), pp.697-709

05/1997

DOI: 10.1046/j.1365-2958.1997.3741745.x

PMID: 9194698

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Abstract

Salmonella typhimurium initiates infection of a host by invading M cells of Peyer's patches within the small intestine. The ability of the bacteria to invade mammalian cells has been shown to be regulated by environmental conditions, including oxygen concentrations, osmolarity, and growth phase. We have previously created oxygen-regulated Tn5lacZY S. typhimurium mutants that are defective in invasion. We have now identified the invasion genes disrupted by eight of the transposon insertions. These genes encode transcriptional regulators (hilA and invF), type III secretory components (orgA, invG and spaR) and secreted proteins (invC and invD). Examination of the protein-secretion profiles of the non-invasive mutants indicated that each of the mutants was defective in secretion of between one and six proteins. We have also demonstrated that the loss of tissue culture cell invasiveness corresponds to an inability to invade and destroy M cells of Peyer's patches in a murine ligated loop model. Virulence studies, performed in mice, demonstrated that these defects significantly reduced the ability of the mutants to cause murine typhoid fever by an oral route of infection. Virulence by an intraperitoneal route of infection was unaffected. The data indicate that in vitro invasiveness, invasion-protein secretion, and M-cell invasion are critical indicators of S. typhimurium virulence.

Details

Title: Subtitle: Non‐invasive Salmonella typhimurium mutants are avirulent because of an inability to enter and destroy M cells of ileal Peyer's patches
Creators: Kristi L Penheiter - Department of Microbiology, University of Iowa School of Medicine, 51 Newton Road, Iowa City, Iowa 52242, USA
Nitin Mathur - Department of Microbiology, University of Iowa School of Medicine, 51 Newton Road, Iowa City, Iowa 52242, USA
Dobie Giles - Department of Microbiology, University of Iowa School of Medicine, 51 Newton Road, Iowa City, Iowa 52242, USA
Thomas Fahlen - Department of Microbiology, University of Iowa School of Medicine, 51 Newton Road, Iowa City, Iowa 52242, USA
Bradley D Jones - Department of Microbiology, University of Iowa School of Medicine, 51 Newton Road, Iowa City, Iowa 52242, USA
Resource Type: Journal article
Publication Details: Molecular microbiology, Vol.24(4), pp.697-709
DOI: 10.1046/j.1365-2958.1997.3741745.x
PMID: 9194698
NLM abbreviation: Mol Microbiol
ISSN: 0950-382X
eISSN: 1365-2958
Language: English
Date published: 05/1997
Academic Unit: Microbiology and Immunology
Record Identifier: 9984083839802771

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