Journal article
Noninvasive detection of hypoxic myocardium using fluorine-18-fluoromisonidazole and positron emission tomography
The Journal of nuclear medicine (1978), Vol.33(12), pp.2202-2208
12/1992
PMID: 1460516
Abstract
Fluoromisonidazole (FMISO) is metabolically trapped in viable cells as a function of reduced cellular pO2. Therefore [18F]-FMISO is potentially useful for evaluating patients with hypoxic but viable myocardium. The goal of this study was to investigate [18F]FMISO uptake in ischemic myocardium non-invasively using positron emission tomography (PET). Studies were performed in 10 open-chest dogs subjected to either complete (Group 1, n = 5) or partial (Group 2, n = 5) occlusion of the left anterior descending coronary artery. The tracer was administered by intravenous bolus following the onset of ischemia and serial PET images were acquired for the next 4 hr. In Group 1, viability was assessed using histochemical staining (nitroblue tetrazolium, NBT) and 99mTc-pyrophosphate (Tc-PYP). In Group 2, viability was assessed using measurements of regional wall motion, histochemical staining and histology (two animals). In each study, PET images obtained at times between 2 and 4 hr postinjection showed specific enhancement of tracer activity in the distal anterior wall and apex of the left ventricle. At 4 hr, the tissue-to-blood pool count ratio was significantly higher in ischemic regions; 1.8 +/- 0.4 for Group 1 and 1.6 +/- 0.2 for Group 2 versus 1.0 +/- 0.1 in nonischemic regions. Postmortem tissue sampling of Group 1 hearts showed significant FMISO retention in samples without evidence for infarction, either by NBT or Tc-PYP deposition, as well as in more severely ischemic regions. In Group 2 animals, FMISO was retained in myocardial regions with reduced blood flow (microspheres), which exhibited improved contraction following reperfusion. We conclude that PET imaging of [18F]FMISO is a promising technique for the noninvasive identification of viable hypoxic myocardium.
Details
- Title: Subtitle
- Noninvasive detection of hypoxic myocardium using fluorine-18-fluoromisonidazole and positron emission tomography
- Creators
- G V Martin - Division of Cardiology, Seattle VA Medical Center, WA 98108J H CaldwellM M GrahamJ R GriersonK KrollM J CowanT K LewellenJ S RaseyJ J CasciariK A Krohn
- Resource Type
- Journal article
- Publication Details
- The Journal of nuclear medicine (1978), Vol.33(12), pp.2202-2208
- Publisher
- United States
- PMID
- 1460516
- ISSN
- 0161-5505
- eISSN
- 1535-5667
- Grant note
- HL38736 / NHLBI NIH HHS P01 CA042045 / NCI NIH HHS CA 42045 / NCI NIH HHS
- Language
- English
- Date published
- 12/1992
- Academic Unit
- Radiology; Radiation Oncology
- Record Identifier
- 9984047881402771
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