Noninvasive real-time measurement of nasal mucociliary clearance in mice by pinhole gamma scintigraphy

Xiaoyang Hua; Kirby L. Zeman; Bingqing Zhou; Qingquan Hua; Brent A. Senior; Stephen L. Tilley; William D. Bennett

doi:10.1152/japplphysiol.00669.2009

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Noninvasive real-time measurement of nasal mucociliary clearance in mice by pinhole gamma scintigraphy

Journal article

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Noninvasive real-time measurement of nasal mucociliary clearance in mice by pinhole gamma scintigraphy

Xiaoyang Hua, Kirby L. Zeman, Bingqing Zhou, Qingquan Hua, Brent A. Senior, Stephen L. Tilley and William D. Bennett

Journal of applied physiology (1985), Vol.108(1), pp.189-196

10/01/2009

DOI: 10.1152/japplphysiol.00669.2009

PMCID: PMC2885071

PMID: 19797687

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Abstract

Mucociliary clearance (MCC) is the key defense mechanism in the upper airways, as the removal of debris-laden mucus in the sinuses completely depends on MCC. So far, how the nasal MCC is regulated remains unknown. Recently, mice deficient in genes encoding the components of MCC apparatus have been generated, which will allow investigators to conduct more in-depth nasal MCC studies. However, the methodology necessary to comprehensively evaluate the nasal MCC in this species is not well established. We therefore developed a novel method to measure nasal MCC in live mice using pinhole gamma camera. Insoluble radiolabeled particles were delivered into the noses of lightly anesthetized mice. The nasal clearance of these particles was measured continuously in a real-time manner. The effect of three different anesthetics—avertin, pentobarbital, and isoflurane—on nasal MCC was also determined. In mice anesthetized by 1.1% isoflurane, radiolabeled particles were immediately moved into the oropharynx, which was significantly accelerated by the treatment of hypertonic but not isotonic saline. According to the clearance rate, the mouse nasal MCC presented two distinct phases: a rapid phase and a slow phase. In addition, we found that isoflurane had a very small inhibitory effect on nasal MCC vs. both avertin and pentobarbital. This was further supported by its dose response. Collectively, we have developed a noninvasive method to monitor the real-time nasal MCC in live mice under physiological conditions. It provides more comprehensive evaluation on nasal MCC rather than assessing a single component of the MCC apparatus in isolation.

anesthesia

avertin

hypertonic saline

Innovative Methodology

isoflurane

pentobarbital

Details

Title: Subtitle: Noninvasive real-time measurement of nasal mucociliary clearance in mice by pinhole gamma scintigraphy
Creators: Xiaoyang Hua - Tongji Medical College
Kirby L. Zeman - Center of Environmental Medicine, Asthma and Lung Biology;
Bingqing Zhou - Department of Biostatistics; and
Qingquan Hua - Wuhan University
Brent A. Senior - University of North Carolina at Chapel Hill
Stephen L. Tilley - Pulmonary Division, Department of Medicine;
William D. Bennett - Pulmonary Division, Department of Medicine;
Resource Type: Journal article
Publication Details: Journal of applied physiology (1985), Vol.108(1), pp.189-196
DOI: 10.1152/japplphysiol.00669.2009
PMID: 19797687
PMCID: PMC2885071
NLM abbreviation: J Appl Physiol (1985)
ISSN: 8750-7587
eISSN: 1522-1601
Publisher: American Physiological Society
Language: English
Date published: 10/01/2009
Academic Unit: Otolaryngology
Record Identifier: 9984312250102771

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