Nonpeptide ligands for human gamma delta T cells

Y Tanaka; S Sano; E Nieves; G De Libero; D Rosa; R L Modlin; M B Brenner; B R Bloom; C T Morita

doi:10.1073/pnas.91.17.8175

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Nonpeptide ligands for human gamma delta T cells

Journal article

Open access

Peer reviewed

Nonpeptide ligands for human gamma delta T cells

Y Tanaka, S Sano, E Nieves, G De Libero, D Rosa, R L Modlin, M B Brenner, B R Bloom and C T Morita

Proceedings of the National Academy of Sciences - PNAS, Vol.91(17), pp.8175-8179

08/16/1994

DOI: 10.1073/pnas.91.17.8175

PMCID: PMC44568

PMID: 8058775

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Abstract

gamma delta T cells respond to a variety of microbial pathogens and transformed cells. Their limited receptor repertoire and activation by mycobacterial antigens resistant to proteases suggest that they may recognize nonpeptide antigens. We have tested a variety of nonpeptide molecules for stimulation of human gamma delta T cells. Synthetic alkyl phosphates, particularly monoethyl phosphate (MEP), selectively activated gamma delta T cells and stimulated their proliferation in vitro. All gamma delta T cells stimulated by MEP expressed V gamma 2/V delta 2 receptors. The purified natural ligand of mycobacteria is chemically similar to, though distinct from, MEP and contains a phosphate residue that is critical for biological activity. Recognition and expansion of a specific T-cell receptor-bearing population to non-peptide ligands is unprecedented among T cells. We suggest that MEP mimics small natural ligands capable of expanding one subset of gamma delta T cells and that this recognition of nonpeptide antigens may play an important role in human immunity to pathogens.

Organophosphates - metabolism

Lymphocyte Activation

Humans

Mycobacterium tuberculosis - immunology

Antigens, Bacterial - pharmacology

Receptors, Antigen, T-Cell, gamma-delta - immunology

Receptors, Antigen, T-Cell, gamma-delta - drug effects

Antibodies - pharmacology

Organophosphates - pharmacology

Synovial Fluid - immunology

Receptors, Antigen, T-Cell, gamma-delta - physiology

Flow Cytometry

T-Lymphocytes - drug effects

Ligands

T-Lymphocytes - immunology

Clone Cells

Arthritis, Rheumatoid - immunology

Cytotoxicity, Immunologic

Details

Title: Subtitle: Nonpeptide ligands for human gamma delta T cells
Creators: Y Tanaka - Howard Hughes Medical Institute, Albert Einstein College of Medicine, Bronx, NY 10461
S Sano
E Nieves
G De Libero
D Rosa
R L Modlin
M B Brenner
B R Bloom
C T Morita
Resource Type: Journal article
Publication Details: Proceedings of the National Academy of Sciences - PNAS, Vol.91(17), pp.8175-8179
DOI: 10.1073/pnas.91.17.8175
PMID: 8058775
PMCID: PMC44568
NLM abbreviation: Proc Natl Acad Sci U S A
ISSN: 0027-8424
eISSN: 1091-6490
Publisher: National Academy of Sciences
Language: English
Date published: 08/16/1994
Academic Unit: Immunology; Internal Medicine
Record Identifier: 9984094714602771

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