Nonrandom chromosome losses in stepwise neoplastic transformation in vitro of human uroepithelial cells

S Q Wu; B E Storer; E A Bookland; A J Klingelhutz; K W Gilchrist; L F Meisner; R Oyasu; C A Reznikoff

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Nonrandom chromosome losses in stepwise neoplastic transformation in vitro of human uroepithelial cells

Journal article

Peer reviewed

Nonrandom chromosome losses in stepwise neoplastic transformation in vitro of human uroepithelial cells

S Q Wu, B E Storer, E A Bookland, A J Klingelhutz, K W Gilchrist, L F Meisner, R Oyasu and C A Reznikoff

Cancer research (Chicago, Ill.), Vol.51(12), pp.3323-3326

06/15/1991

PMID: 2040006

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Abstract

An in vitro/in vivo transformation system was used to study chromosome region losses in stepwise neoplastic transformation and progression of human uroepithelial cells. Complete cytogenetic analyses were done on 17 independent carcinomas derived using this system and showed that losses of chromosome regions on 3p (P = 0.0003), 6q (P = 0.01), and 18q (P = 0.0003) were nonrandom. The smallest common losses [i.e., 3(p13---pter), 6(q21---q23), and 18(q21.1---qter)] were in putative cancer suppressor gene regions. In addition, cumulative losses from a group of 10 chromosome arms (i.e., 1p, 1q, 3p, 5q, 6q, 9q, 11p, 13q, 17p, and 18q) frequently deleted in clinical carcinomas were very significant (P = 0.0005) compared to losses from all other arms. Loss of 3p and 18q both correlated with transformation to high grade carcinomas (P = 0.001 and P = 0.004, respectively). These data provide new evidence supporting hypotheses that chromosome regions 3(p13---pter) and 6(q21---q23) contain genes that suppress cancer development. These results also provide new data confirming the hypothesis that genetic loss(es) in the 18(q21.1---qter) region are associated with the development of high grade malignancies.

Chromosome Deletion

Cell Line

Chromosome Banding

Urinary Bladder Neoplasms - genetics

Cell Transformation, Neoplastic

Humans

Karyotyping

Chromosomes, Human

Epithelium

Genes, Tumor Suppressor

Urinary Bladder

Carcinoma, Transitional Cell - genetics

Details

Title: Subtitle: Nonrandom chromosome losses in stepwise neoplastic transformation in vitro of human uroepithelial cells
Creators: S Q Wu - University of Wisconsin Clinical Cancer Center, Departments of Human Oncology, Madison
B E Storer
E A Bookland
A J Klingelhutz
K W Gilchrist
L F Meisner
R Oyasu
C A Reznikoff
Resource Type: Journal article
Publication Details: Cancer research (Chicago, Ill.), Vol.51(12), pp.3323-3326
PMID: 2040006
NLM abbreviation: Cancer Res
ISSN: 0008-5472
eISSN: 1538-7445
Publisher: United States
Grant note: R01-29525-12 / PHS HHS P01-BM144-CA13-1 / NCI NIH HHS
Language: English
Date published: 06/15/1991
Academic Unit: Microbiology and Immunology; Radiation Oncology
Record Identifier: 9984001155302771

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