Journal article
Norepinephrine increases alveolar fluid reabsorption and Na, K-ATPase activity
American journal of respiratory and critical care medicine, Vol.170(7), pp.730-736
2004
DOI: 10.1164/rccm.200308-1127OC
PMID: 15256395
Abstract
The purpose of this study was to determine whether α-adrenergic receptor agonists have a role in alveolar fluid reabsorption, via Na,K-ATPase, in the alveolar epithelium. Alveolar fluid reabsorption increased approximately twofold with increasing concentrations of norepinephrine (NE) as compared with control rats. Treatment with the nonselective α-adrenergic receptor agonist, octopamine, and the specific a, agonist, phenylephrine, increased alveolar fluid reabsorption by 54 and 40%, respectively, as compared with control. The specific α1,-adrenergic receptor antagonist, prazosin, inhibited the stimulatory effects of NE by approximately 30%, whereas α2-adrenergic antagonist, yohimbine, did not prevent the stimulatory effects of NE. The administration of ouabain, Na,K-ATPase inhibitor, prevented the NE-mediated increase in alveolar fluid reabsorption. In parallel with these changes, NE increased Na,K-ATPase activity and protein abundance in alveolar epithelial type II cells via the α1- and β-adrenergic receptor. We report here that NE increased alveolar fluid reabsorption via the activation of both α1,- and β-adrenergic receptors, but not α2-adrenergic receptors. These effects are due to increased activity and abundance of the Na,K-ATPase in the basolateral membrane of ATII cells.
Details
- Title: Subtitle
- Norepinephrine increases alveolar fluid reabsorption and Na, K-ATPase activity
- Creators
- Zaher S Azzam - Veterans Health AdministrationYochai Adir - Northwestern UniversityAstrid Crespo - Northwestern UniversityAlejandro Comellas - Northwestern UniversityEmilia LecuonaLaura A DadaNorberto Krivoy - Northwestern UniversityDavid H Rutschman - Northwestern UniversityJacob I SznajderKaren M Ridge
- Resource Type
- Journal article
- Publication Details
- American journal of respiratory and critical care medicine, Vol.170(7), pp.730-736
- Publisher
- American Lung Association
- DOI
- 10.1164/rccm.200308-1127OC
- PMID
- 15256395
- ISSN
- 1073-449X
- eISSN
- 1535-4970
- Language
- English
- Date published
- 2004
- Academic Unit
- Pulmonary, Critical Care, and Occupational Medicine; ICTS; Internal Medicine
- Record Identifier
- 9984359808402771
Metrics
10 Record Views