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Notch1 in primary effusion lymphoma: a clinicopathological study
Journal article   Open access   Peer reviewed

Notch1 in primary effusion lymphoma: a clinicopathological study

Huan-You Wang, Franklin S Fuda, Weina Chen and Nitin J Karandikar
Modern pathology, Vol.23(6), pp.773-780
06/2010
DOI: 10.1038/modpathol.2010.67
PMID: 20348880
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https://doi.org/10.1038/modpathol.2010.67View
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Abstract

Primary effusion lymphoma is a human herpes virus 8 (HHV-8)-associated large cell lymphoma of body cavities. Detailed large-scale clinicopathological studies are rarely reported, and the underlying mechanism of lymphomagenesis remains elusive. In the present report, we studied the clinicodemographic, immunophenotypic, and cytomorphological features on a cohort of 12 cases of primary effusion lymphoma. In contrast to HHV-8, which was positive in all nine cases tested (100%), HIV was found in 75% (9/12) of cases, whereas the three HIV-negative cases were either in elderly patients (one with hepatitis C virus infection and one with asbestoses exposure) or in a heart transplantation recipient. By flow cytometry, the antigens expressed in descending order were CD38, CD71, HLA-DR, CD30, and CD45RO. B-cell markers were largely negative. Cytomorphologically, all cases showed atypical to anaplastic morphology. Notch1, a member of transmembrane signal transduction family, was found in six of seven HHV-8-positive cases (86%). In agreement with in vitro studies using human primary effusion lymphoma cell lines, we have found that Notch1 was expressed in the majority of HHV-8-positive primary effusion lymphoma cases, corroborating the notion that Notch1 may have an important role in HHV-8-mediated lymphomagenesis of primary effusion lymphoma.
 Immunohistochemistry Flow Cytometry Polymerase Chain Reaction Membrane Glycoproteins - analysis ADP-ribosyl Cyclase 1 - analysis Herpesvirus 4, Human - genetics Lymphoma, Primary Effusion - chemistry Prognosis Humans Middle Aged DNA, Viral - analysis Lymphoma, Primary Effusion - virology Male Antigens, CD - analysis Herpesvirus 8, Human - isolation & purification In Situ Hybridization Leukocyte Common Antigens - analysis Ki-1 Antigen - analysis Aged, 80 and over Adult Female Receptor, Notch1 - analysis Genes, T-Cell Receptor Herpesvirus 8, Human - genetics Immunophenotyping Lymphoma, Primary Effusion - pathology HIV - isolation & purification Receptors, Transferrin - analysis Lymphoma, Primary Effusion - immunology Gene Rearrangement HLA-DR Antigens - analysis Aged Immunoglobulin Heavy Chains - genetics

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