Journal article
Novel Quantitative Biosystem for Modeling Physiological Fluid Shear Stress on Cells
Applied and environmental microbiology, Vol.73(3), pp.699-705
02/2007
DOI: 10.1128/AEM.02428-06
PMCID: PMC1800738
PMID: 17142365
Abstract
The response of microbes to changes in the mechanical force of fluid shear has important implications for pathogens, which experience wide fluctuations in fluid shear in vivo during infection. However, the majority of studies have not cultured microbes under physiological fluid shear conditions within a range commonly encountered by microbes during host-pathogen interactions. Here we describe a convenient batch culture biosystem in which (i) the levels of fluid shear force can be varied within physiologically relevant ranges and quantified via mathematical models and (ii) large numbers of cells can be planktonically grown and harvested to examine the effect of fluid shear levels on microbial genomic and phenotypic responses. A quantitative model based on numerical simulations and in situ imaging analysis was developed to calculate the fluid shear imparted by spherical beads of different sizes on bacterial cell cultures grown in a rotating wall vessel (RWV) bioreactor. To demonstrate the application of this model, we subjected cultures of the bacterial pathogen
Salmonella enterica
serovar Typhimurium to three physiologically-relevant fluid shear ranges during growth in the RVW and demonstrated a progressive relationship between the applied fluid shear and the bacterial genetic and phenotypic responses. By applying this model to different cell types, including other bacterial pathogens, entire classes of genes and proteins involved in cellular interactions may be discovered that have not previously been identified during growth under conventional culture conditions, leading to new targets for vaccine and therapeutic development.
Details
- Title: Subtitle
- Novel Quantitative Biosystem for Modeling Physiological Fluid Shear Stress on Cells
- Creators
- Eric A Nauman - School of Mechanical Engineering, Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana 47907-2088C. Mark Ott - School of Mechanical Engineering, Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana 47907-2088Ed Sander - School of Mechanical Engineering, Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana 47907-2088Don L Tucker - School of Mechanical Engineering, Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana 47907-2088Duane Pierson - School of Mechanical Engineering, Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana 47907-2088James W Wilson - School of Mechanical Engineering, Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana 47907-2088Cheryl A Nickerson - School of Mechanical Engineering, Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana 47907-2088
- Resource Type
- Journal article
- Publication Details
- Applied and environmental microbiology, Vol.73(3), pp.699-705
- DOI
- 10.1128/AEM.02428-06
- PMID
- 17142365
- PMCID
- PMC1800738
- NLM abbreviation
- Appl Environ Microbiol
- ISSN
- 0099-2240
- eISSN
- 1098-5336
- Publisher
- American Society for Microbiology
- Language
- English
- Date published
- 02/2007
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Orthopedics and Rehabilitation; Craniofacial Anomalies Research Center; Chemical and Biochemical Engineering
- Record Identifier
- 9984064208002771
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