Novel Staphylococcus aureus Secreted Protein Alters Keratinocyte Proliferation and Elicits a Proinflammatory Response In Vitro and In Vivo

Joseph A Merriman; Aloysius J Klingelhutz; Daniel J Diekema; Donald Y M Leung; Patrick M Schlievert

doi:10.1021/acs.biochem.5b00523

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Novel Staphylococcus aureus Secreted Protein Alters Keratinocyte Proliferation and Elicits a Proinflammatory Response In Vitro and In Vivo

Journal article

Peer reviewed

Novel Staphylococcus aureus Secreted Protein Alters Keratinocyte Proliferation and Elicits a Proinflammatory Response In Vitro and In Vivo

Joseph A Merriman, Aloysius J Klingelhutz, Daniel J Diekema, Donald Y M Leung and Patrick M Schlievert

Biochemistry (Easton), Vol.54(31), pp.4855-4862

08/11/2015

DOI: 10.1021/acs.biochem.5b00523

PMCID: PMC4912018

PMID: 26177220

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https://www.ncbi.nlm.nih.gov/pmc/articles/4912018View

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Abstract

Staphylococcus aureus is a leading cause of surgical site infections that results in increased hospital stays due to the development of chronic wounds. Little is known about factors involved in S. aureus' ability to prevent wounds from healing. We discovered a novel secreted protein produced by a surgical site isolate of S. aureus that prevents keratinocyte proliferation. The protein has a molecular weight of 15.7 kDa and an isoelectric point of 8.9. The cloned and purified protein has cytotoxic and proinflammatory properties, as shown in vitro and in vivo. Potent biological effects on keratinocytes and rabbit skin suggest that this protein may play an important role in preventing re-epithelialization. Its lack of homology to known exotoxins suggests that this protein is novel, and this observation is likely to open a new field of research in S. aureus exotoxins. Due to its cytotoxic activities, we call this new protein ε-cytotoxin.

Staphylococcus aureus - genetics

Inflammation - pathology

Rabbits

Inflammation - microbiology

Cell Proliferation

Humans

Bacterial Proteins - genetics

Staphylococcal Skin Infections - metabolism

Staphylococcal Skin Infections - pathology

Staphylococcus aureus - pathogenicity

Inflammation - metabolism

Keratinocytes - pathology

Animals

Keratinocytes - metabolism

Inflammation - genetics

Bacterial Proteins - metabolism

Cell Line, Transformed

Staphylococcal Skin Infections - genetics

Staphylococcus aureus - metabolism

Details

Title: Subtitle: Novel Staphylococcus aureus Secreted Protein Alters Keratinocyte Proliferation and Elicits a Proinflammatory Response In Vitro and In Vivo
Creators: Joseph A Merriman
Aloysius J Klingelhutz
Daniel J Diekema
Donald Y M Leung - §University of Colorado, Denver, Anschutz Medical Campus, Aurora, Colorado 80045, United States
Patrick M Schlievert
Resource Type: Journal article
Publication Details: Biochemistry (Easton), Vol.54(31), pp.4855-4862
DOI: 10.1021/acs.biochem.5b00523
PMID: 26177220
PMCID: PMC4912018
NLM abbreviation: Biochemistry
ISSN: 1520-4995
eISSN: 1520-4995
Publisher: United States
Grant note: U19 AI117673 / NIAID NIH HHS 1 U19 AI117673-01 / NIAID NIH HHS
Language: English
Date published: 08/11/2015
Academic Unit: Microbiology and Immunology; Infectious Diseases; Pathology; Radiation Oncology; Internal Medicine
Record Identifier: 9983986270002771

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