Novel animal model for Achilles tendinopathy: Controlled experimental study of serial injections of collagenase in rabbits

Cesar de Cesar Netto; Alexandre Leme Godoy-Santos; Pedro Augusto Pontin; Renato Jose Mendonça Natalino; Cesar Augusto Martins Pereira; Francisco Diego de Oliveira Lima; Lucas Furtado da Fonseca; Jackson Rucker Staggers; Leonardo Muntada Cavinatto; Lew Charles Schon; Olavo Pires de Camargo; Túlio Diniz Fernandes

doi:10.1371/journal.pone.0192769

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Novel animal model for Achilles tendinopathy: Controlled experimental study of serial injections of collagenase in rabbits

Journal article

Open access

Peer reviewed

Novel animal model for Achilles tendinopathy: Controlled experimental study of serial injections of collagenase in rabbits

Cesar de Cesar Netto, Alexandre Leme Godoy-Santos, Pedro Augusto Pontin, Renato Jose Mendonça Natalino, Cesar Augusto Martins Pereira, Francisco Diego de Oliveira Lima, Lucas Furtado da Fonseca, Jackson Rucker Staggers, Leonardo Muntada Cavinatto, Lew Charles Schon, …

PloS one, Vol.13(2), pp.e0192769-e0192769

2018

DOI: 10.1371/journal.pone.0192769

PMCID: PMC5811024

PMID: 29438431

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Abstract

Our goal was to develop a novel technique for inducing Achilles tendinopathy in animal models which more accurately represents the progressive histological and biomechanical characteristic of chronic Achilles tendinopathy in humans. In this animal research study, forty-five rabbits were randomly assigned to three groups and given bilateral Achilles injections. Low dose (LD group) (n = 18) underwent a novel technique with three low-dose (0.1mg) injections of collagenase that were separated by two weeks, the high dose group (HD) (n = 18) underwent traditional single high-dose (0.3mg) injections, and the third group were controls (n = 9). Six rabbits were sacrificed from each experimental group (LD and HD) at 10, 12 and 16 weeks. Control animals were sacrificed after 16 weeks. Histological and biomechanical properties were then compared in all three groups. At 10 weeks, Bonar score and tendon cross sectional area was highest in HD group, with impaired biomechanical properties compared to LD group. At 12 weeks, Bonar score was higher in LD group, with similar biomechanical findings when compared to HD group. After 16 weeks, Bonar score was significantly increased for both LD group (11,8±2,28) and HD group (5,6±2,51), when compared to controls (2±0,76). LD group showed more pronounced histological and biomechanical findings, including cross sectional area of the tendon, Young's modulus, yield stress and ultimate tensile strength. In conclusion, Achilles tendinopathy in animal models that were induced by serial injections of low-dose collagenase showed more pronounced histological and biomechanical findings after 16 weeks than traditional techniques, mimicking better the progressive and chronic characteristic of the tendinopathy in humans.

Collagenases - administration & dosage

Rabbits

Biomechanical Phenomena

Tendinopathy - pathology

Achilles Tendon - pathology

Animals

Female

Tendinopathy - chemically induced

Disease Models, Animal

Details

Title: Subtitle: Novel animal model for Achilles tendinopathy: Controlled experimental study of serial injections of collagenase in rabbits
Creators: Cesar de Cesar Netto - Department of Orthopaedics, Foot and Ankle Surgery, Hospital for Special Surgery (HSS), New York, New York, United States of America
Alexandre Leme Godoy-Santos - Department of Orthopaedics and Traumatology, University of Sao Paulo (USP), School of Medicine, Sao Paulo, Sao Paulo, Brazil
Pedro Augusto Pontin - Department of Orthopaedics and Traumatology, University of Sao Paulo (USP), School of Medicine, Sao Paulo, Sao Paulo, Brazil
Renato Jose Mendonça Natalino - Department of Orthopaedics and Traumatology, University of Sao Paulo (USP), School of Medicine, Sao Paulo, Sao Paulo, Brazil
Cesar Augusto Martins Pereira - Department of Orthopaedics and Traumatology, University of Sao Paulo (USP), School of Medicine, Sao Paulo, Sao Paulo, Brazil
Francisco Diego de Oliveira Lima - Department of Orthopaedics and Traumatology, University of Sao Paulo (USP), School of Medicine, Sao Paulo, Sao Paulo, Brazil
Lucas Furtado da Fonseca - Department of Orthopaedics, Foot and Ankle Surgery, Medstar Union Memorial Hospital, Baltimore, Maryland, United States of America
Jackson Rucker Staggers - Department of Orthopaedics, University of Alabama at Birmingham (UAB), Birmingham, Alabama, United States of America
Leonardo Muntada Cavinatto - Department of Orthopaedics and Traumatology, University of Sao Paulo (USP), School of Medicine, Sao Paulo, Sao Paulo, Brazil
Lew Charles Schon - Department of Orthopaedics, Foot and Ankle Surgery, Medstar Union Memorial Hospital, Baltimore, Maryland, United States of America
Olavo Pires de Camargo - Department of Orthopaedics and Traumatology, University of Sao Paulo (USP), School of Medicine, Sao Paulo, Sao Paulo, Brazil
Túlio Diniz Fernandes - Department of Orthopaedics and Traumatology, University of Sao Paulo (USP), School of Medicine, Sao Paulo, Sao Paulo, Brazil
Resource Type: Journal article
Publication Details: PloS one, Vol.13(2), pp.e0192769-e0192769
DOI: 10.1371/journal.pone.0192769
PMID: 29438431
PMCID: PMC5811024
NLM abbreviation: PLoS One
ISSN: 1932-6203
eISSN: 1932-6203
Publisher: Public Library of Science; United States
Grant note: DOI: 10.13039/501100001807, name: FAPESP, award: 2013/2504813
Language: English
Date published: 2018
Academic Unit: Orthopedics and Rehabilitation
Record Identifier: 9984040464902771

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