Journal article
Novel methods for adenovirus-mediated gene transfer to blood vessels in vivo
Molecular and cellular biochemistry, Vol.172(1-2), pp.37-46
07/1997
DOI: 10.1023/A:1006803202179
PMID: 9278230
Abstract
Adenovirus-mediated gene transfer is a promising method for studies of vascular biology and potentially for gene therapy. Intravascular approaches for gene transfer to blood vessels in vivo generally require interruption of blood flow and have several limitations. We have used two alternative approaches for gene transfer to blood vessels in vivo using perivascular application of vectors. First, replication-deficient adenovirus expressing nuclear-targeted bacterial beta-galactosidase was injected into cerebrospinal fluid via the cisterna magna of rats. Leptomeningeal cells over the major arteries were efficiently transfected, and adventitial cells of large vessels and smooth muscle cells of small vessels were occasionally stained. When viral suspension was injected with the rat in a lateral position, the reporter gene was expressed extensively on the ipsilateral surface of the brain. Thus, adenovirus injected into cerebrospinal fluid provides gene transfer in vivo to cerebral blood vessels and, with greater efficiency, to perivascular tissue. Furthermore, positioning of the head may 'target' specific regions of the brain. Second, vascular gene delivery was accomplished by perivascular injection of virus in peripheral vessels. Injection of the adenoviral vector within the periarterial sheath of monkeys resulted in gene transfer to the vessel wall that was substantial in magnitude although limited to cells in the adventitia. Approximately 20% of adventitial cells expressed the transgene, with no gene transfer to cells in the intima or media. These approaches may provide alternative approaches for gene transfer to blood vessels, and may be useful for studies of vascular biology and perhaps vascular gene therapy.
Details
- Title: Subtitle
- Novel methods for adenovirus-mediated gene transfer to blood vessels in vivo
- Creators
- H Ooboshi - Department of Internal Medicine, University of Iowa College of Medicine, Veterans Administration Medical Center, Iowa City 52242, USAC D RíosD D Heistad
- Resource Type
- Journal article
- Publication Details
- Molecular and cellular biochemistry, Vol.172(1-2), pp.37-46
- Publisher
- Netherlands
- DOI
- 10.1023/A:1006803202179
- PMID
- 9278230
- ISSN
- 0300-8177
- eISSN
- 1573-4919
- Grant note
- NS 24621 / NINDS NIH HHS HL160669 / NHLBI NIH HHS HL 14388 / NHLBI NIH HHS
- Language
- English
- Date published
- 07/1997
- Academic Unit
- Cardiovascular Medicine; Neuroscience and Pharmacology; Internal Medicine
- Record Identifier
- 9984040470002771
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