Journal article
Novel quasi-subgenotype D2 of hepatitis B virus identified in Taiwanese aborigines
Virus genes, Vol.49(1), pp.30-37
08/2014
DOI: 10.1007/s11262-014-1072-x
PMID: 24792512
Abstract
The hepatitis B virus (HBV) genomes in Taiwanese aborigines, whose ancestors have lived in Taiwan for over 10,000 years, have not been characterized. In order to characterize of HBV in this special population, serum samples were obtained from serologically HBsAg-positive 27 Taiwanese aborigines. The pre-S1/S2 region and the full-length 3.2 kb of the HBV genome were amplified by PCR. Obtained amplicons were sequenced and confirmed the HBV genotypes by phylogenetic analysis. By phylogenetic analysis of the sequence of pre-S1/pre-S2 region, HBV/B2 (21/27: 78 %) was the most prevalent followed by genotype D (6/27: 22 %). Two strains of HBV/B2, each having 3,215 bp genomes, had recombination with genotype C in the pre-C/C gene which is characteristic of subgenotype B2 circulating in Southeast Asia. Interestingly, six strains of genotype D formed a distinct cluster between subgenotypes D1 and D2 suggesting a novel group of HBV. A similar finding could also be confirmed based on the entire 3,182 bp genome from four strains of HBV/D. This new cluster was supported by a branch with 99 % bootstrap value and 3.4–5.8 % nucleotide divergence over the entire genome from other known subgenotypes D1 to D9. Four strains of the new D subgenotype showed serotype ayw2, but had unique amino acid sequences consisting of N115 in the preS/S gene; P41 in the X gene; S239, K/E295, V567, and P708 in the P gene, respectively. From the above results, we provisionally proposed to designate it as novel quasi-subgenotype D2 identified in Taiwanese aborigines.
Details
- Title: Subtitle
- Novel quasi-subgenotype D2 of hepatitis B virus identified in Taiwanese aborigines
- Creators
- Huy Tran - Department of Pathology National Institute of Infectious Diseases Toyama 1-23-1 Shinjuku-ku Tokyo 162-8640 JapanMing-Lung Yu - Faculty of Internal Medicine, School of Medicine, College of Medicine Kaohsiung Medical University Kaohsiung TaiwanChia-Yen Dai - Faculty of Internal Medicine, School of Medicine, College of Medicine Kaohsiung Medical University Kaohsiung TaiwanI-Ling Lin - Department of Medical Laboratory Science and Biotechnology, College of Health Sciences Kaohsiung Medical University Kaohsiung TaiwanMing-Lun Yeh - Hepatobiliary Division, Department of Internal Medicine and Hepatitis Center Kaohsiung Medical University Hospital Kaohsiung TaiwanWan-Long Chuang - Taiwan Liver Research Foundation Kaohsiung TaiwanKenji Abe - Department of Pathology National Institute of Infectious Diseases Toyama 1-23-1 Shinjuku-ku Tokyo 162-8640 Japan
- Resource Type
- Journal article
- Publication Details
- Virus genes, Vol.49(1), pp.30-37
- Publisher
- Springer US
- DOI
- 10.1007/s11262-014-1072-x
- PMID
- 24792512
- ISSN
- 0920-8569
- eISSN
- 1572-994X
- Language
- English
- Date published
- 08/2014
- Academic Unit
- Gastroenterology and Hepatology; Internal Medicine
- Record Identifier
- 9984094541802771
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