Journal article
Nox2 Deficiency Prevents Hypertension-Induced Vascular Dysfunction and Hypertrophy in Cerebral Arterioles
International journal of hypertension, Vol.2013, pp.1-8
2013
DOI: 10.1155/2013/793630
PMCID: PMC3612447
PMID: 23573415
Abstract
Oxidative stress is involved in many hypertension-related vascular diseases in the brain, including stroke and dementia. Thus, we examined the role of genetic deficiency of NADPH oxidase subunit Nox2 in the function and structure of cerebral arterioles during hypertension. Arterial pressure was increased in right-sided cerebral arterioles with transverse aortic banding for 4 weeks in 8-week-old wild-type (WT) and Nox2-deficient (-/y) mice. Mice were givenNG-nitro-L-arginine methyl ester (L-NAME, 10 mg/kg) or vehicle to drink. We measured the reactivity in cerebral arterioles through open cranial window in anesthetized mice and wall cross-sectional area and superoxide levelsex vivo. Aortic constriction increased systolic and pulse pressures in right-sided carotid arteries in all groups of mice. Ethidium fluorescence showed increased superoxide in right-sided cerebral arterioles in WT, but not in Nox2-/y mice. Dilation to acetylcholine, but not sodium nitroprusside, was reduced, and cross-sectional areas were increased in the right-sided arterioles in WT, but were unchanged in Nox2-/y mice. L-NAME reduced dilation to acetylcholine but did not result in hypertrophy in right-sided arterioles of Nox2-/y mice. In conclusion, hypertension-induced superoxide production derived from Nox2-containing NADPH oxidase promotes hypertrophy and causes endothelial dysfunction in cerebral arterioles, possibly involving interaction with nitric oxide.
Details
- Title: Subtitle
- Nox2 Deficiency Prevents Hypertension-Induced Vascular Dysfunction and Hypertrophy in Cerebral Arterioles
- Creators
- Siu-Lung Chan - University of VermontGary L Baumbach - University of Vermont
- Resource Type
- Journal article
- Publication Details
- International journal of hypertension, Vol.2013, pp.1-8
- DOI
- 10.1155/2013/793630
- PMID
- 23573415
- PMCID
- PMC3612447
- NLM abbreviation
- Int J Hypertens
- ISSN
- 2090-0384
- eISSN
- 2090-0392
- Grant note
- DOI: 10.13039/100000968, name: American Heart Association, award: 0725668Z, HL-22149, NS72628, NS24621, NS-62461
- Language
- English
- Date published
- 2013
- Academic Unit
- Pathology; Fraternal Order of Eagles Diabetes Research Center
- Record Identifier
- 9984186618702771
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