Journal article
Nrf2 deficiency in myeloid cells is not sufficient to protect mice from high-fat diet-induced adipose tissue inflammation and insulin resistance
Free radical biology & medicine, Vol.52(9), pp.1708-1715
05/01/2012
DOI: 10.1016/j.freeradbiomed.2012.02.022
PMCID: PMC3383807
PMID: 22370093
Abstract
Activation of the transcription factor NF-E2-related factor 2 (Nrf2) by oxidative stress induces the expression of a variety of antioxidant and anti-inflammatory genes. Yet, genetic ablation of Nrf2 was shown to protect mice from high-fat diet (HFD)-induced obesity and insulin resistance. The mechanisms that underlie this seemingly paradoxical finding remain largely unexplored. Here we examined whether Nrf2 deficiency in myeloid cells contributes to protection against HFD-induced metabolic changes by decreasing adipose tissue inflammation. In vitro, induction of IL-1 beta by inflammatory stimuli was significantly reduced in Nrf2-deficient macrophages. Whereas inflammatory gene expression in the stromal vascular fraction was reduced in both global and chimeric Nrf2 KO mice, only global Nrf2-deficient, and not bone marrow-transplanted Nrf2 chimeric, mice were protected against HFD-induced adipose tissue inflammation. Whereas global Nrf2 deficiency resulted in significantly decreased expression of inflammatory genes and PPAR gamma 2, there was no difference when Nrf2 was absent only from myeloid cells. In vitro coculture with adipocytes demonstrated that macrophage Nrf2 regulated inflammatory gene expression in macrophages; however, it was not required to induce inflammatory gene expression in adipocytes. Finally, in contrast to global Nrf2 knockout, Nrf2 deficiency in myeloid cells did not protect against HFD-induced insulin resistance. Together, our data demonstrate a dominant role for nonmyeloid Nrf2 in controlling HFD-induced adipose tissue inflammation and the development of insulin resistance. (c) 2012 Elsevier Inc. All rights reserved.
Details
- Title: Subtitle
- Nrf2 deficiency in myeloid cells is not sufficient to protect mice from high-fat diet-induced adipose tissue inflammation and insulin resistance
- Creators
- Akshaya K. Meher - University of VirginiaPoonam R. Sharma - University of VirginiaVitor A. Lira - University of VirginiaMasayuki Yamamoto - Tohoku UniversityThomas W. Kensler - University of PittsburghZhen Yan - University of VirginiaNorbert Leitinger - University of Virginia
- Resource Type
- Journal article
- Publication Details
- Free radical biology & medicine, Vol.52(9), pp.1708-1715
- DOI
- 10.1016/j.freeradbiomed.2012.02.022
- PMID
- 22370093
- PMCID
- PMC3383807
- NLM abbreviation
- Free Radic Biol Med
- ISSN
- 0891-5849
- eISSN
- 1873-4596
- Publisher
- Elsevier
- Number of pages
- 8
- Grant note
- R01-HL-084422-01 / National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA R01AR050429 / NATIONAL INSTITUTE OF ARTHRITIS AND MUSCULOSKELETAL AND SKIN DISEASES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Arthritis & Musculoskeletal & Skin Diseases (NIAMS) R01CA094076 / NATIONAL CANCER INSTITUTE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI) R01HL084422 / NATIONAL HEART, LUNG, AND BLOOD INSTITUTE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Heart Lung & Blood Institute (NHLBI) 09POST2210019 / American Heart Association
- Language
- English
- Date published
- 05/01/2012
- Academic Unit
- Health, Sport, and Human Physiology
- Record Identifier
- 9984259651202771
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