Nuclear envelope breakdown induced by herpes simplex virus type 1 involves the activity of viral fusion proteins

Martina Maric; Alison C Haugo; William Dauer; David Johnson; Richard J Roller

doi:10.1016/j.virol.2014.05.010

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Nuclear envelope breakdown induced by herpes simplex virus type 1 involves the activity of viral fusion proteins

Journal article

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Nuclear envelope breakdown induced by herpes simplex virus type 1 involves the activity of viral fusion proteins

Martina Maric, Alison C Haugo, William Dauer, David Johnson and Richard J Roller

Virology (New York, N.Y.), Vol.460-461(1), pp.128-137

07/2014

DOI: 10.1016/j.virol.2014.05.010

PMCID: PMC4101023

PMID: 25010278

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Abstract

Herpesvirus infection reorganizes components of the nuclear lamina usually without loss of integrity of the nuclear membranes. We report that wild-type HSV infection can cause dissolution of the nuclear envelope in transformed mouse embryonic fibroblasts that do not express torsinA. Nuclear envelope breakdown is accompanied by an eight-fold inhibition of virus replication. Breakdown of the membrane is much more limited during infection with viruses that lack the gB and gH genes, suggesting that breakdown involves factors that promote fusion at the nuclear membrane. Nuclear envelope breakdown is also inhibited during infection with virus that does not express UL34, but is enhanced when the US3 gene is deleted, suggesting that envelope breakdown may be enhanced by nuclear lamina disruption. Nuclear envelope breakdown cannot compensate for deletion of the UL34 gene suggesting that mixing of nuclear and cytoplasmic contents is insufficient to bypass loss of the normal nuclear egress pathway. •We show that wild-type HSV can induce breakdown of the nuclear envelope in a specific cell system.•The viral fusion proteins gB and gH are required for induction of nuclear envelope breakdown.•Nuclear envelope breakdown cannot compensate for deletion of the HSV UL34 gene.

Fusion

HSV-1

US3

Nuclear envelope

UL34

Details

Title: Subtitle: Nuclear envelope breakdown induced by herpes simplex virus type 1 involves the activity of viral fusion proteins
Creators: Martina Maric - Department of Microbiology, University of Iowa, Iowa City, IA 52242, USA
Alison C Haugo - Department of Microbiology, University of Iowa, Iowa City, IA 52242, USA
William Dauer - Department of Neurology, University of Michigan, Ann Arbor, MI 48109, USA
David Johnson - Department of Microbiology and Immunology, Oregon Health Sciences University, Portland, OR 97201, USA
Richard J Roller - Department of Microbiology, University of Iowa, Iowa City, IA 52242, USA
Resource Type: Journal article
Publication Details: Virology (New York, N.Y.), Vol.460-461(1), pp.128-137
DOI: 10.1016/j.virol.2014.05.010
PMID: 25010278
PMCID: PMC4101023
NLM abbreviation: Virology
ISSN: 0042-6822
eISSN: 1096-0341
Publisher: Elsevier Inc
Grant note: DOI: 10.13039/100000002, name: NIH, award: R21 AI099597
Language: English
Date published: 07/2014
Academic Unit: Microbiology and Immunology
Record Identifier: 9984001135602771

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