Nuclear factor 1 and T-cell factor/LEF recognition elements regulate Pitx2 transcription in pituitary development

Di Ai; Jun Wang; Melanie Amen; Mei-Fang Lu; Brad A Amendt; James F Martin

doi:10.1128/MCB.01848-06

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Nuclear factor 1 and T-cell factor/LEF recognition elements regulate Pitx2 transcription in pituitary development

Journal article

Open access

Peer reviewed

Nuclear factor 1 and T-cell factor/LEF recognition elements regulate Pitx2 transcription in pituitary development

Di Ai, Jun Wang, Melanie Amen, Mei-Fang Lu, Brad A Amendt and James F Martin

Molecular and cellular biology, Vol.27(16), pp.5765-5775

08/2007

DOI: 10.1128/MCB.01848-06

PMCID: PMC1952127

PMID: 17562863

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https://europepmc.org/articles/pmc1952127View

Published (Version of record) Open Access

Abstract

Pitx2, a paired-related homeobox gene that is mutated in Rieger syndrome I, is the earliest known marker of oral ectoderm. Pitx2 was previously shown to be required for tooth, palate, and pituitary development in mice; however, the mechanisms regulating Pitx2 transcription in the oral ectoderm are poorly understood. Here we used an in vivo transgenic approach to investigate the mechanisms regulating Pitx2 transcription. We identified a 7-kb fragment that directs LacZ expression in oral ectoderm and in many of its derivatives. Deletion analysis of transgenic embryos reduced this fragment to a 520-bp region that directed LacZ activity to Rathke's pouch. A comparison of the mouse and human sequences revealed a conserved nuclear factor 1 (NF-1) recognition element near a consensus T-cell factor (TCF)/LEF binding site. The mutation of either site individually abolished LacZ activity in transgenic embryos, identifying Pitx2 as a direct target of Wnt signaling in pituitary development. These findings uncover a requirement for NF-1 and TCF factors in Pitx2 transcriptional regulation in the pituitary and provide insight into the mechanisms controlling region-specific transcription in the oral ectoderm and its derivatives.

3' Flanking Region - genetics

Animals

Base Sequence

Binding Sites

Ectoderm - cytology

Ectoderm - metabolism

Embryo, Mammalian - cytology

Embryo, Mammalian - metabolism

Enhancer Elements, Genetic - genetics

Gene Expression Regulation, Developmental

Homeodomain Proteins - genetics

Humans

Mice

Models, Genetic

Molecular Sequence Data

Mouth - embryology

Mutation - genetics

NFI Transcription Factors - metabolism

Pituitary Gland - embryology

Protein Binding

TCF Transcription Factors - metabolism

Transcription Factors - genetics

Transcription, Genetic

Details

Title: Subtitle: Nuclear factor 1 and T-cell factor/LEF recognition elements regulate Pitx2 transcription in pituitary development
Creators: Di Ai - Texas A&M University
Jun Wang - Texas A&M University
Melanie Amen - Texas A&M University
Mei-Fang Lu - Texas A&M University
Brad A Amendt - Texas A&M University
James F Martin - Texas A&M University
Resource Type: Journal article
Publication Details: Molecular and cellular biology, Vol.27(16), pp.5765-5775
DOI: 10.1128/MCB.01848-06
PMID: 17562863
PMCID: PMC1952127
NLM abbreviation: Mol Cell Biol
ISSN: 0270-7306
eISSN: 1098-5549
Grant note: R01 DE013941 / NIDCR NIH HHS DE13941 / NIDCR NIH HHS R01 DE016329 / NIDCR NIH HHS R01 DE16329-01 / NIDCR NIH HHS
Language: English
Date published: 08/2007
Academic Unit: Orthodontics; Anatomy and Cell Biology; Craniofacial Anomalies Research Center; Dental Research
Record Identifier: 9984284460002771

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