Journal article
Nuclear magnetic resonance structural studies of a potassium channel-charybdotoxin complex
Biochemistry (Easton), Vol.44(48), pp.15834-15841
12/06/2005
DOI: 10.1021/bi051656d
PMID: 16313186
Abstract
Ion channels play critical roles in signaling processes and are attractive targets for treating various diseases. Here we describe an NMR-based strategy for structural analyses of potassium channel-ligand complexes using KcsA (residues 1-132, with six mutations to impart toxin binding and to mimic the eukaryotic hERG channel). Using this approach, we determined the solution structure of KcsA in complex with the high-affinity peptide antagonist charybdotoxin. The structural data reveal how charybdotoxin binds to the closed form of KcsA and makes specific contacts with the extracellular surface of the ion channel, resulting in pore blockage. This represents the first direct structural information about an ion channel complexed to a peptide antagonist and provides an experimental framework for understanding and interpreting earlier mutational analyses. The strategy presented here overcomes many of the limitations of conventional NMR approaches to helical membrane protein structure determination and can be applied in the study of the binding of druglike molecules to this important class of proteins.
Details
- Title: Subtitle
- Nuclear magnetic resonance structural studies of a potassium channel-charybdotoxin complex
- Creators
- Liping Yu - AbbottChaohong Sun - AbbottDanying Song - AbbottJianwei ShenNan Xu - AbbottAngelo Gunasekera - AbbottPhilip J Hajduk - AbbottEdward T Olejniczak - Abbott
- Resource Type
- Journal article
- Publication Details
- Biochemistry (Easton), Vol.44(48), pp.15834-15841
- DOI
- 10.1021/bi051656d
- PMID
- 16313186
- ISSN
- 0006-2960
- eISSN
- 1520-4995
- Language
- English
- Date published
- 12/06/2005
- Academic Unit
- Biochemistry and Molecular Biology; Medicine Administration
- Record Identifier
- 9984627214702771
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