Journal article
Nucleotide sequence and expression of the human gene encoding apolipoprotein H (β2-glycoprotein I)
Gene, Vol.108(2), pp.293-298
1991
DOI: 10.1016/0378-1119(91)90449-L
PMID: 1748314
Abstract
Human apolipoprotein H (ApoH), also called β2-glycoprotein I, is a 50-kDa serum glycoprotein whose function is not clearly defined. We have cloned and sequenced
ApoH cDNAs both from human liver and from a human hepatoma cell line (HepG2). Both cDNA sequences predict a protein 345 amino acids (aa) in length. This sequence includes a 19-aa hydrophobic, N-terminal signal sequence which is not present in the mature protein [Lozier et al., Proc. Natl. Acad. Sci. USA 81 (1984) 3640–3644]. It differs from this previously reported aa sequence at two positions, both of which strengthen the conservation among the four short consensus repeats within the ApoH molecule. COS-1 cells transiently transfected with the
ApoH cDNA in a eukaryotic expression vector produced a single species of
ApoH mRNA and secreted in the ApoH protein. The level of
ApoH mRNA expressed by HepG2 cells is downregulated by incubation with inflammatory mediators, implying that ApoH is a negative acute-phase protein.
Details
- Title: Subtitle
- Nucleotide sequence and expression of the human gene encoding apolipoprotein H (β2-glycoprotein I)
- Creators
- Haider Mehdi - Rush University Medical CenterMichael Nunn - CytelDiana M. Steel - Harvard Medical SchoolAlexander S. Whitehead - Harvard Medical SchoolMary Perez - CytelLes Walker - CytelMark E. Peeples - Rush University Medical Center
- Resource Type
- Journal article
- Publication Details
- Gene, Vol.108(2), pp.293-298
- Publisher
- Elsevier B.V
- DOI
- 10.1016/0378-1119(91)90449-L
- PMID
- 1748314
- ISSN
- 0378-1119
- eISSN
- 1879-0038
- Language
- English
- Date published
- 1991
- Academic Unit
- Cardiovascular Medicine; Internal Medicine
- Record Identifier
- 9984359952702771
Metrics
4 Record Views