Nucleotide sequences and biologic properties of toxic shock syndrome toxin 1 from ovine- and bovine-associated Staphylococcus aureus

Peter K Lee; Barry N Kreiswirth; James R Deringer; Steven J Projan; William Eisner; Beverly L Smith; Eunice Carlson; Richard P Novick; Patrick M Schlievert

doi:10.1093/infdis/165.6.1056

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Nucleotide sequences and biologic properties of toxic shock syndrome toxin 1 from ovine- and bovine-associated Staphylococcus aureus

Journal article

Peer reviewed

Nucleotide sequences and biologic properties of toxic shock syndrome toxin 1 from ovine- and bovine-associated Staphylococcus aureus

Peter K Lee, Barry N Kreiswirth, James R Deringer, Steven J Projan, William Eisner, Beverly L Smith, Eunice Carlson, Richard P Novick and Patrick M Schlievert

The Journal of infectious diseases, Vol.165(6), pp.1056-1063

06/1992

DOI: 10.1093/infdis/165.6.1056

PMID: 1583323

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Abstract

Toxic shock syndrome toxin (TSST) 1 was purified from ovine (TSST-ovine) and bovine (TSST-bovine) mastitis-associated Staphylococcus aureus. These toxins were previously reported to have molecular weights identical to that of human TSST-1. However, TSST-ovine was reported as having an isoelectric point (pI) of 8.5, whereas TSST-bovine has the same pI (7.2) as TSST-1. Nucleotide sequence analysis revealed that TSST-bovine was identical to TSST-1 and that TSST-ovine had 14 nucleotide differences that changed 9 amino acid residues. Only 1 nucleotide difference, at position 514, was predicted to cause an amino acid charge difference, as glutamic acid at position 132 of TSST-1 was changed to lysine in TSST-ovine. Like TSST-1, TSST-ovine was mitogenic, but unlike TSST-1, it was not pyrogenic, was unable to enhance endotoxic shock, and was unable to induce TSS in a rabbit model. Also, TSST-ovine was less reactive to certain monoclonal antibodies raised against TSST-1.

Amino Acid Sequence

Rabbits

Shock, Septic - etiology

Sheep Diseases - microbiology

Lymphocyte Activation

Humans

Molecular Sequence Data

DNA, Bacterial - chemistry

Blotting, Western

Mastitis - microbiology

Staphylococcal Infections - veterinary

Mastitis, Bovine - microbiology

Enterotoxins - genetics

Animals

Mastitis - veterinary

Bacterial Toxins

Cattle

Base Sequence

Superantigens

Female

Sheep

Staphylococcal Infections - microbiology

Enterotoxins - chemistry

Staphylococcus aureus

Details

Title: Subtitle: Nucleotide sequences and biologic properties of toxic shock syndrome toxin 1 from ovine- and bovine-associated Staphylococcus aureus
Creators: Peter K Lee - Department of Microbiology, University of Minnesota Medical School, Minneapolis 55455-0312
Barry N Kreiswirth
James R Deringer
Steven J Projan
William Eisner
Beverly L Smith
Eunice Carlson
Richard P Novick
Patrick M Schlievert
Resource Type: Journal article
Publication Details: The Journal of infectious diseases, Vol.165(6), pp.1056-1063
Publisher: United States
DOI: 10.1093/infdis/165.6.1056
PMID: 1583323
ISSN: 0022-1899
eISSN: 1537-6613
Grant note: CA-09138 / NCI NIH HHS AI-22159 / NIAID NIH HHS
Language: English
Date published: 06/1992
Academic Unit: Microbiology and Immunology; Internal Medicine
Record Identifier: 9984001120102771

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