Nucleotide sequences that signal the initiation of transcription and translation in Bacillus subtilis

C P Moran Jr; N Lang; S F LeGrice; G Lee; Michael Stephens; A L Sonenshein; Janice Pero; Richard Losick

doi:10.1007/BF00729452

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Nucleotide sequences that signal the initiation of transcription and translation in Bacillus subtilis

Journal article

Peer reviewed

Nucleotide sequences that signal the initiation of transcription and translation in Bacillus subtilis

C P Moran Jr, N Lang, S F LeGrice, G Lee, Michael Stephens, A L Sonenshein, Janice Pero and Richard Losick

Molecular & general genetics, Vol.186(3), pp.339-346

1982

DOI: 10.1007/BF00729452

PMID: 6181373

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Abstract

We have determined the nucleotide sequence of two Bacillus subtilis promoters (veg and tms) that are utilized by the principal form of B. subtilis RNA polymerase found in vegetative cells (sigma 55-RNA polymerase) and have compared our sequences to those of several previously reported Bacillus promoters. Hexanucleotide sequences centered approximately 35 (the "--35" region) and 10 (the "--10" region) base pairs upstream from the veg and tms transcription starting points (and separated by 17 base pairs) corresponded closely to the consensus hexanucleotides (TTGACA and TATAAT) attributed to Escherichia coli promoters. Conformity to the preferred --35 and --10 sequences may not be sufficient to promote efficient utilization by B. subtilis RNA polymerase, however, since three promoters (veg, tms and E. coli tac) that conform to these sequences and that are utilized efficiently by E. coli RNA polymerase were used with highly varied efficiencies by B. subtilis RNA polymerase. We have also analyzed mRNA sequences in DNA located downstream from eight B. subtilis chromosomal and phage promoters for nucleotide sequences that might signal the initiation of translation. In accordance with the rules of McLaughlin, Murray and Rabinowitz (1981), we observe mRNA nucleotide sequences with extensive complementarity to the 3' terminal region of B. subtilis 16S rRNA, followed by an initiation codon and an open reading frame.

Genes, Bacterial

Protein Biosynthesis

Operon

Base Sequence

RNA, Messenger - genetics

RNA, Bacterial - genetics

Ribosomes - metabolism

Transcription, Genetic

DNA-Directed RNA Polymerases - genetics

Binding Sites

Bacillus subtilis - metabolism

Bacillus subtilis - genetics

Details

Title: Subtitle: Nucleotide sequences that signal the initiation of transcription and translation in Bacillus subtilis
Creators: C P Moran Jr
N Lang
S F LeGrice
G Lee
Michael Stephens
A L Sonenshein
Janice Pero
Richard Losick
Resource Type: Journal article
Publication Details: Molecular & general genetics, Vol.186(3), pp.339-346
Publisher: Germany
DOI: 10.1007/BF00729452
PMID: 6181373
ISSN: 0026-8925
eISSN: 1432-1874
Grant note: GM19168 / NIGMS NIH HHS GM18568 / NIGMS NIH HHS
Language: English
Date published: 1982
Academic Unit: Iowa Neuroscience Institute; Immunology; Internal Medicine
Record Identifier: 9984065378302771

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