Journal article
O-antigen and Core Carbohydrate of Vibrio fischeri Lipopolysaccharide: COMPOSITION AND ANALYSIS OF THEIR ROLE IN EUPRYMNA SCOLOPES LIGHT ORGAN COLONIZATION
The Journal of biological chemistry, Vol.287(11), pp.8515-8530
03/09/2012
DOI: 10.1074/jbc.M111.324012
PMCID: PMC3318738
PMID: 22247546
Abstract
Background:
The structure and function of the
Vibrio fischeri
O-antigen were unknown.
Results:
The O-antigen structure was determined using an O-antigen ligase mutant.
Conclusion:
This mutant had a motility defect, and comparison of its LPS with wild-type LPS showed that the O-antigen contains some unusual sugars.
Significance:
The O-antigen mutation results in a significantly slower rate of bacterial colonization of the squid light organ.
Vibrio fischeri
exists in a symbiotic relationship with the Hawaiian bobtail squid,
Euprymna scolopes
, where the squid provides a home for the bacteria, and the bacteria in turn provide camouflage that helps protect the squid from night-time predators. Like other Gram-negative organisms,
V. fischeri
expresses lipopolysaccharide (LPS) on its cell surface. The structure of the O-antigen and the core components of the LPS and their possible role in colonization of the squid have not previously been determined. In these studies, an O-antigen ligase mutant,
waaL
, was utilized to determine the structures of these LPS components and their roles in colonization of the squid. WaaL ligates the O-antigen to the core of the LPS; thus, LPS from
waaL
mutants lacks O-antigen. Our results show that the
V. fischeri waaL
mutant has a motility defect, is significantly delayed in colonization, and is unable to compete with the wild-type strain in co-colonization assays. Comparative analyses of the LPS from the wild-type and
waaL
strains showed that the
V. fischeri
LPS has a single O-antigen repeat composed of yersiniose, 8-epi-legionaminic acid, and
N
-acetylfucosamine. In addition, the LPS from the
waaL
strain showed that the core structure consists of
l
-glycero-
d
-manno-heptose,
d
-glycero-
d
-manno-heptose, glucose, 3-deoxy-
d
-manno-octulosonic acid,
N
-acetylgalactosamine, 8-epi-legionaminic acid, phosphate, and phosphoethanolamine. These studies indicate that the unusual
V. fischeri
O-antigen sugars play a role in the early phases of bacterial colonization of the squid.
Details
- Title: Subtitle
- O-antigen and Core Carbohydrate of Vibrio fischeri Lipopolysaccharide: COMPOSITION AND ANALYSIS OF THEIR ROLE IN EUPRYMNA SCOLOPES LIGHT ORGAN COLONIZATION
- Creators
- Deborah M. B. Post - Buck Institute for Research on AgingLiping Yu - University of IowaBenjamin C. Krasity - University of Wisconsin–MadisonBiswa Choudhury - University of California, San DiegoMark J. Mandel - Northwestern UniversityCaitlin A. Brennan - University of Wisconsin–MadisonEdward G. Ruby - University of Wisconsin–MadisonMargaret J. McFall-Ngai - University of Wisconsin–MadisonBradford W. Gibson - University of California, San FranciscoMichael A. Apicella - University of Iowa
- Resource Type
- Journal article
- Publication Details
- The Journal of biological chemistry, Vol.287(11), pp.8515-8530
- Publisher
- American Society for Biochemistry and Molecular Biology
- DOI
- 10.1074/jbc.M111.324012
- PMID
- 22247546
- PMCID
- PMC3318738
- ISSN
- 0021-9258
- eISSN
- 1083-351X
- Grant note
- PO1 AI44642; RO1 AI24616; R01 AI50661; R01 RR 12294; IOS 0817232 / National Institutes of Health
- Alternative title
- Structure and Function of Vibrio fischeri O-antigen
- Language
- English
- Date published
- 03/09/2012
- Academic Unit
- Microbiology and Immunology; Biochemistry and Molecular Biology; Medicine Administration
- Record Identifier
- 9984627324702771
Metrics
3 Record Views