Journal article
OMIP-099: 31-color spectral flow cytometry panel to investigate the steady-state phenotype of human T cells
Cytometry. Part A, Vol.105(1), pp.10-15
01/2024
DOI: 10.1002/cyto.a.24799
PMCID: PMC10842108
PMID: 37814476
Appears in UI Libraries Support Open Access
Abstract
We have developed a 31-color panel to define the steady-state phenotype of T cells in human peripheral blood (Table 1). The panel presented here was optimized using cryopreserved peripheral blood mononuclear cells (PBMC). The markers included in this panel were chosen in order to characterize the steady-state phenotype of T cells and includes markers (CD45RA, CD45RO, CCR7, CD95) to distinguish the main subsets (e.g., naïve, TEM, TCM, TEMRA, TSCM etc.) of CD4, CD8, and γδ T cells. This panel also includes markers for the identification of differentiation status (CD27, CD28), activation/antigen experience status (CD11a, CD49d, CD38, HLA-DR, CD56, and CD39), co-inhibitory marker expression (PD-1, TIM-3), and CD4 T helper subsets (CXCR3, CXCR5, CCR4, CCR6, Foxp3, CD25, and CD127). This optimized panel provides a broad assessment of the steady-state phenotype of human T cells.
Details
- Title: Subtitle
- OMIP-099: 31-color spectral flow cytometry panel to investigate the steady-state phenotype of human T cells
- Creators
- Zeb R Zacharias - University of IowaJon C D Houtman - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Cytometry. Part A, Vol.105(1), pp.10-15
- DOI
- 10.1002/cyto.a.24799
- PMID
- 37814476
- PMCID
- PMC10842108
- NLM abbreviation
- Cytometry A
- eISSN
- 1552-4930
- Publisher
- Wiley
- Grant note
- DOI: 10.13039/100000002, name: National Institutes of Health
- Language
- English
- Electronic publication date
- 10/09/2023
- Date published
- 01/2024
- Academic Unit
- Microbiology and Immunology; Holden Comprehensive Cancer Center; Internal Medicine
- Record Identifier
- 9984476558402771
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