Journal article
Obesity alters immune and metabolic profiles: New insight from obese‐resistant mice on high‐fat diet
Obesity (Silver Spring, Md.), Vol.24(10), pp.2140-2149
10/2016
DOI: 10.1002/oby.21620
PMCID: PMC5039085
PMID: 27515998
Abstract
Objective
Diet‐induced obesity has been shown to alter immune function in mice, but distinguishing the effects of obesity from changes in diet composition is complicated. It was hypothesized that immunological differences would exist between diet‐induced obese (DIO) and obese‐resistant (OB‐Res) mice fed the same high‐fat diet (HFD).
Methods
BALB/c mice were fed either standard chow or HFD to generate lean or DIO and OB‐Res mice, respectively. Resulting mice were analyzed for serum immunologic and metabolic profiles and cellular immune parameters.
Results
BALB/c mice on HFD were categorized as DIO or OB‐Res, based on body weight versus lean controls. DIO mice were physiologically distinct from OB‐Res mice, whose serum insulin, leptin, gastric inhibitory polypeptide, and eotaxin concentrations remained similar to lean controls. DIO mice had increased macrophage+ crown‐like structures in white adipose tissue, although macrophage percentages were unchanged from OB‐Res and lean mice. DIO mice also had decreased splenic CD4+ T cells, elevated serum GM‐CSF, and increased splenic CD11c+ dendritic cells, but impaired dendritic cell stimulatory capacity (P < 0.05 vs. lean controls). These parameters were unaltered in OB‐Res mice versus lean controls.
Conclusions
Diet‐induced obesity results in alterations in immune and metabolic profiles that are distinct from effects caused by HFD alone.
Details
- Title: Subtitle
- Obesity alters immune and metabolic profiles: New insight from obese‐resistant mice on high‐fat diet
- Creators
- Shannon K Boi - Graduate Biomedical Sciences, University of Alabama at BirminghamClaire M Buchta - The University of Iowa Carver College of MedicineNicole A Pearson - The Obesity Research and Education Initiative, and the Fraternal Order of Eagles' Diabetes Research Center, The University of Iowa Carver College of MedicineMeghan B Francis - University of Alabama at BirminghamDavid K Meyerholz - The University of Iowa Carver College of MedicineJustin L Grobe - The Obesity Research and Education Initiative, and the Fraternal Order of Eagles' Diabetes Research Center, The University of Iowa Carver College of MedicineLyse A Norian - Nutrition Obesity Research Center and Comprehensive Cancer Center, University of Alabama at Birmingham
- Resource Type
- Journal article
- Publication Details
- Obesity (Silver Spring, Md.), Vol.24(10), pp.2140-2149
- DOI
- 10.1002/oby.21620
- PMID
- 27515998
- PMCID
- PMC5039085
- ISSN
- 1930-7381
- eISSN
- 1930-739X
- Number of pages
- 10
- Grant note
- American Diabetes Association (1‐14‐BS‐079) NIH (HL084207) NIH (R01CA181088) NCI Cancer Prevention and Control Training Program Fellowship 5‐R25‐CA47888 (CA47888)
- Language
- English
- Date published
- 10/2016
- Academic Unit
- Pathology; Neuroscience and Pharmacology
- Record Identifier
- 9984083269302771
Metrics
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