Journal article
Object-location training elicits an overlapping but temporally distinct transcriptional profile from contextual fear conditioning
Neurobiology of learning and memory, Vol.116, pp.90-95
12/2014
DOI: 10.1016/j.nlm.2014.09.002
PMCID: PMC4262591
PMID: 25242102
Abstract
[Display omitted]
•Microfluidic technology allows for large-scale qPCR experiments.•Object-location memory increases IEG expression similarly to fear conditioning.•Object-location memory induces gene expression changes that last at least 2h.
Hippocampus-dependent learning is known to induce changes in gene expression, but information on gene expression differences between different learning paradigms that require the hippocampus is limited. The bulk of studies investigating RNA expression after learning use the contextual fear conditioning task, which couples a novel environment with a footshock. Although contextual fear conditioning has been useful in discovering gene targets, gene expression after spatial memory tasks has received less attention. In this study, we used the object-location memory task and studied gene expression at two time points after learning in a high-throughput manner using a microfluidic qPCR approach. We found that expression of the classic immediate-early genes changes after object-location training in a fashion similar to that observed after contextual fear conditioning. However, the temporal dynamics of gene expression are different between the two tasks, with object-location memory producing gene expression changes that last at least 2hours. Our findings indicate that different training paradigms may give rise to distinct temporal dynamics of gene expression after learning.
Details
- Title: Subtitle
- Object-location training elicits an overlapping but temporally distinct transcriptional profile from contextual fear conditioning
- Creators
- Shane G Poplawski - Pharmacology Graduate Group, University of Pennsylvania, Philadelphia, PA 19104, USAHannah Schoch - Cell and Molecular Biology Graduate Group, University of Pennsylvania, Philadelphia, PA 19104, USAMathieu E Wimmer - Department of Psychiatry, University of Pennsylvania, Philadelphia, PA 19104, USAJoshua D Hawk - Department of Cell Biology, Yale University, New Haven, CT 06520, USAJennifer L Walsh - Departments of Psychology and Mathematics, Swarthmore College, Swarthmore, PA 19081, USAKarl P Giese - Centre for the Cellular Basis of Behaviour, King’s College London, London SE5 9NU, UKTed Abel - Pharmacology Graduate Group, University of Pennsylvania, Philadelphia, PA 19104, USA
- Resource Type
- Journal article
- Publication Details
- Neurobiology of learning and memory, Vol.116, pp.90-95
- DOI
- 10.1016/j.nlm.2014.09.002
- PMID
- 25242102
- PMCID
- PMC4262591
- NLM abbreviation
- Neurobiol Learn Mem
- ISSN
- 1074-7427
- eISSN
- 1095-9564
- Publisher
- Elsevier BV
- Language
- English
- Date published
- 12/2014
- Academic Unit
- Molecular Physiology and Biophysics; Psychiatry; Psychological and Brain Sciences; Iowa Neuroscience Institute; Neuroscience and Pharmacology; Biochemistry and Molecular Biology
- Record Identifier
- 9984065737402771
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