Octachloronaphthalene induction of hepatic microsomal aryl hydrocarbon hydroxylase activity in the immature male rat

M A Campbell; S Bandiera; L Robertson; A Parkinson; S Safe

doi:10.1016/0300-483X(81)90112-8

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Octachloronaphthalene induction of hepatic microsomal aryl hydrocarbon hydroxylase activity in the immature male rat

Journal article

Peer reviewed

Octachloronaphthalene induction of hepatic microsomal aryl hydrocarbon hydroxylase activity in the immature male rat

M A Campbell, S Bandiera, L Robertson, A Parkinson and S Safe

Toxicology (Amsterdam), Vol.22(2), pp.123-132

1981

DOI: 10.1016/0300-483X(81)90112-8

PMID: 7324072

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Abstract

Administration of octachloronaphthalene to immature male Wistar rats resulted in a dose-dependent increase in several enzymic, electrophoretic and spectral parameters associated with induction of the hepatic microsomal enzymes. Compared to corn-oil (control)treated animals octachloronaphthalene (150 mumol . kg-1) induced hepatic cytochrome P-450 (1.5-fold), benzo [alpha] pyrene hydroxylase (18-fold) and 4-chlorobiphenyl hydroxylase (18-fold) enzyme activities. In addition to increases in the relative peak intensities of the reduced microsomal cytochrome P-450 : CO and ethylisocyanide (EIC) difference spectra the peak maxima were observed at 448.5 and 452.2/428.0 nm, respectively. The effects of administering octachloronaphthalene to the rat were similar to those observed after pretreatment with 3-methylcholanthrene (MC) and electrophoresis of the induced microsomal proteins showed that both compounds enhance heme-staining peptides with comparable electrophoretic mobilities. Moreover coadministration of MC (3 x 100 mumol . kg-1) and octachloronaphthalene (2 x 150 mumol . kg-1) indicated that their inductive effects were not additive. It was concluded that octachloronaphthalene was an MC-type inducer of hepatic microsomal enzymes.

Age Factors

Animals

Aryl Hydrocarbon Hydroxylases - biosynthesis

Drug Interactions

Enzyme Induction - drug effects

Hydrocarbons, Chlorinated - toxicity

In Vitro Techniques

Male

Methylcholanthrene - pharmacology

Microsomes, Liver - drug effects

Microsomes, Liver - enzymology

Phenobarbital - pharmacology

Rats

Rats, Inbred Strains

Details

Title: Subtitle: Octachloronaphthalene induction of hepatic microsomal aryl hydrocarbon hydroxylase activity in the immature male rat
Creators: M A Campbell - University of Guelph
S Bandiera - University of Guelph
L Robertson - University of Guelph
A Parkinson - University of Guelph
S Safe - University of Guelph
Resource Type: Journal article
Publication Details: Toxicology (Amsterdam), Vol.22(2), pp.123-132
DOI: 10.1016/0300-483X(81)90112-8
PMID: 7324072
ISSN: 0300-483X
eISSN: 1879-3185
Language: English
Date published: 1981
Academic Unit: Occupational and Environmental Health
Record Identifier: 9984364439702771

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