Journal article
Octopamine receptor OAMB is required for ovulation in Drosophila melanogaster
Developmental biology, Vol.264(1), pp.179-190
2003
DOI: 10.1016/j.ydbio.2003.07.018
PMID: 14623240
Abstract
Octopamine is a major monoamine in invertebrates and affects many physiological processes ranging from energy metabolism to complex behaviors. Octopamine binds to receptors located on various cell types and activates distinct signal transduction pathways to produce these diverse effects. We previously identified one of the
Drosophila octopamine receptors named OAMB that produces increases in cAMP and intracellular Ca
2+ upon ligand binding. It is expressed at high levels in the brain. To explore OAMB's physiological roles, we generated deletions in the OAMB locus. The resultant
oamb mutants were viable without gross anatomical defects. The
oamb females displayed normal courtship and copulation; however, they were impaired in ovulation with many mature eggs retained in their ovaries. RT-PCR, in situ hybridization, and expression of a reporter gene revealed that OAMB was also expressed in the thoracicoabdominal ganglion, the female reproductive system, and mature eggs in the ovary. Moreover, analysis of various alleles pinpointed the requirement for OAMB in the body, but not in the brain, for female fecundity. The novel expression pattern of OAMB and its genetic resource described in this study will help advance our understanding on how the neuromodulatory or endocrine system controls reproductive physiology and behavior.
Details
- Title: Subtitle
- Octopamine receptor OAMB is required for ovulation in Drosophila melanogaster
- Creators
- Hyun-Gwan Lee - Department of Biobehavioral Health and The Huck Institute of Life Sciences, 315 Health and Human Development East, Pennsylvania State University, University Park, PA 16802, USAChang-Soo Seong - Department of Biobehavioral Health and The Huck Institute of Life Sciences, 315 Health and Human Development East, Pennsylvania State University, University Park, PA 16802, USAYoung-Cho Kim - Department of Biobehavioral Health and The Huck Institute of Life Sciences, 315 Health and Human Development East, Pennsylvania State University, University Park, PA 16802, USARonald L Davis - Departments of Molecular and Cell Biology, and Psychiatry and Behavioral Sciences, Baylor College of Medicine, Houston, TX 77030, USAKyung-An Han - Department of Biobehavioral Health and The Huck Institute of Life Sciences, 315 Health and Human Development East, Pennsylvania State University, University Park, PA 16802, USA
- Resource Type
- Journal article
- Publication Details
- Developmental biology, Vol.264(1), pp.179-190
- DOI
- 10.1016/j.ydbio.2003.07.018
- PMID
- 14623240
- NLM abbreviation
- Dev Biol
- ISSN
- 0012-1606
- eISSN
- 1095-564X
- Publisher
- Elsevier Inc
- Language
- English
- Date published
- 2003
- Academic Unit
- Neurology; Iowa Neuroscience Institute
- Record Identifier
- 9984020727602771
Metrics
18 Record Views