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Ocular neovascularization. Tissue culture studies
Journal article   Peer reviewed

Ocular neovascularization. Tissue culture studies

X Q Gu, G L Fry, G F Lata, A J Packer, E G Servais, J C Hoak and S S Hayreh
Archives of ophthalmology (1960), Vol.103(1), pp.111-117
01/1985
DOI: 10.1001/archopht.1985.01050010117033
PMID: 2579627

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Abstract

The proliferative activity of a number of intraocular fluids, bovine retinal extract, and normal serum (from humans and cynomolgus monkeys) was investigated by in vitro tissue culture studies, with the use of tritiated thymidine incorporation by the cultured endothelial cells of human umbilical veins. There was increased tritiated thymidine incorporation by (1) the aqueous, vitreous, and intraocular fluid (IOF) (which filled the eye after lensectomy and vitrectomy) removed from cynomolgus monkey eyes with iris neovascularization or with neovascular glaucoma (NVG) that developed after experimental retinal vein occlusion, (2) by aqueous and vitreous removed from human eyes with NVG or proliferative diabetic retinopathy; (3) by the serum, and (4) by the bovine retinal extract. However, tritiated thymidine incorporation was not increased by the normal aqueous, vitreous, or IOF.
Constriction Culture Techniques Humans Lens, Crystalline - surgery Macaca fascicularis Retinal Vein Vitreous Body - physiology Retina - physiology Animals Cattle Neovascularization, Pathologic - physiopathology Vitrectomy Tissue Extracts Aqueous Humor - physiology Glaucoma - physiopathology Iris - blood supply

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