Journal article
Oleic Acid Incorporation Protects Cultured Hamster Fibroblasts from Oxygen-Induced Cytotoxicity
The Journal of nutrition, Vol.126(12), pp.2952-2959
12/01/1996
DOI: 10.1093/jn/126.12.2952
PMID: 9001361
Abstract
The effects of oleic, linoleic and arachidonic acid on oxygen toxicity were evaluated in cultured hamster fibroblasts. Each fatty acid was incorporated separately using a protocol that resulted in dose-dependent increases in the respective cellular fatty acid content to as much as 20- fold greater than unsupplemented controls. Linoleic acid produced no changes in cell survival after 48 h treatment with 95% oxygen, regardless of fatty acid content of the cells. Oleic acid incorporation resulted in a dose- dependent increase in cell survival at 48 h in 95% oxygen, whereas arachidonic acid incorporation resulted in a dose-dependent decrease in cell survival at 48 h in 95% oxygen. No significant differences in amounts of linoleic or arachidonic acids were detected in control, oleic acid- enriched or linoleic acid-enriched cells during oxygen exposure. In cells enriched with arachidonic acid, exposure to oxygen significantly reduced the amounts of linoleic and arachidonic acid to 79 and 84%, respectively, of the amounts found in air-exposed cells. The results indicate that oleic acid incorporation into cells provides protection against 95% oxygen-induced cytotoxicity. In contrast, arachidonic acid incorporation led to sensitization of cells to 95% oxygen-induced cytotoxicity that was accompanied by a loss of polyunsaturated fatty acids. As a result, it would appear that in situations of increased oxidative stress, high monounsaturated fatty acid diets that increase cellular oleic acid content may provide a protective environment compared with high polyunsaturated fatty acid diets that increase cellular arachidonic acid content.
Details
- Title: Subtitle
- Oleic Acid Incorporation Protects Cultured Hamster Fibroblasts from Oxygen-Induced Cytotoxicity
- Creators
- Michael Kinter - University of VirginiaDouglas R. Spitz - MallinckrodtRobert J. Robert - Department of Pediatrics, University of Virginia Health Sciences Center, Charlottesville, VA 22908
- Resource Type
- Journal article
- Publication Details
- The Journal of nutrition, Vol.126(12), pp.2952-2959
- DOI
- 10.1093/jn/126.12.2952
- PMID
- 9001361
- ISSN
- 0022-3166
- eISSN
- 1541-6100
- Language
- English
- Date published
- 12/01/1996
- Academic Unit
- Pathology; Radiation Oncology; Fraternal Order of Eagles Diabetes Research Center
- Record Identifier
- 9984312981302771
Metrics
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