On-demand biomanufacturing of protective conjugate vaccines

Jessica C Stark; Thapakorn Jaroentomeechai; Tyler D Moeller; Jasmine M Hershewe; Katherine F Warfel; Bridget S Moricz; Anthony M Martini; Rachel S Dubner; Karen J Hsu; Taylor C Stevenson; Bradley D Jones; Matthew P DeLisa; Michael C Jewett

doi:10.1126/sciadv.abe9444

Back

On-demand biomanufacturing of protective conjugate vaccines

Journal article

Open access

Peer reviewed

On-demand biomanufacturing of protective conjugate vaccines

Jessica C Stark, Thapakorn Jaroentomeechai, Tyler D Moeller, Jasmine M Hershewe, Katherine F Warfel, Bridget S Moricz, Anthony M Martini, Rachel S Dubner, Karen J Hsu, Taylor C Stevenson, …

Science advances, Vol.7(6), eabe9444

02/2021

DOI: 10.1126/sciadv.abe9444

PMCID: PMC7857678

PMID: 33536221

Files and links (1)

url

https://doi.org/10.1126/sciadv.abe9444View

Published (Version of record) Open Access

Abstract

Conjugate vaccines are among the most effective methods for preventing bacterial infections. However, existing manufacturing approaches limit access to conjugate vaccines due to centralized production and cold chain distribution requirements. To address these limitations, we developed a modular technology for in vitro conjugate vaccine expression (iVAX) in portable, freeze-dried lysates from detoxified, nonpathogenic Upon rehydration, iVAX reactions synthesize clinically relevant doses of conjugate vaccines against diverse bacterial pathogens in 1 hour. We show that iVAX-synthesized vaccines against subsp. (type A) strain Schu S4 protected mice from lethal intranasal challenge. The iVAX platform promises to accelerate development of new conjugate vaccines with increased access through refrigeration-independent distribution and portable production.

Details

Title: Subtitle: On-demand biomanufacturing of protective conjugate vaccines
Creators: Jessica C Stark - Northwestern Technological Institute
Thapakorn Jaroentomeechai - Cornell University
Tyler D Moeller - Cornell University
Jasmine M Hershewe - Northwestern Technological Institute
Katherine F Warfel - Northwestern Technological Institute
Bridget S Moricz - University of Iowa
Anthony M Martini - University of Iowa
Rachel S Dubner - Northwestern University
Karen J Hsu - Northwestern Technological Institute
Taylor C Stevenson - Cornell University
Bradley D Jones - University of Iowa
Matthew P DeLisa - Cornell University
Michael C Jewett - Northwestern Technological Institute
Resource Type: Journal article
Publication Details: Science advances, Vol.7(6), eabe9444
DOI: 10.1126/sciadv.abe9444
PMID: 33536221
PMCID: PMC7857678
NLM abbreviation: Sci Adv
ISSN: 2375-2548
eISSN: 2375-2548
Grant note: T32 EB023860 / NIBIB NIH HHS
Language: English
Date published: 02/2021
Academic Unit: Microbiology and Immunology
Record Identifier: 9984297321002771

Metrics

15 Record Views

83 Times Cited - Web of Science