On-line monitoring of human prostate cancer cells in a perfusion rotating wall vessel by near-infrared spectroscopy

Martin H Rhiel; Michael B Cohen; Mark A Arnold; David W Murhammer

doi:10.1002/bit.10834

Back

On-line monitoring of human prostate cancer cells in a perfusion rotating wall vessel by near-infrared spectroscopy

Journal article

Open access

Peer reviewed

On-line monitoring of human prostate cancer cells in a perfusion rotating wall vessel by near-infrared spectroscopy

Martin H Rhiel, Michael B Cohen, Mark A Arnold and David W Murhammer

Biotechnology and bioengineering, Vol.86(7), pp.852-861

06/30/2004

DOI: 10.1002/bit.10834

PMID: 15162462

Files and links (1)

url

https://doi.org/10.1002/bit.10834View

Published (Version of record) Open Access

Abstract

PC-3 human prostate cancer cells have been cultivated in a rotating wall vessel in which glucose, lactate, and glutamine profiles were monitored noninvasively and in real time by near-infrared (NIR) spectroscopy. The calibration models were based on off-line spectra from tissue culture experiments described previously (Rhiel et al., Biotechnol Bioeng 77:73-82). Monitoring performance was improved by Fourier filtering of the spectra and initial off-set adjustment. The resulting standard errors of predictions were 0.95, 0.74, and 0.39 mM for glucose, lactate, and glutamine, respectively. The concentration of ammonia could not be accurately measured from the same spectra. In addition, metabolite uptake and production rates were determined for PC-3 prostate cancer cells during exponential growth in batch-mode cultivation. Cells grew with a doubling time of 21 h and consumed glucose and glutamine at rates of 6.8 and 1.8 x 10(-17) mol/cell.s, respectively. This resulted in lactate and ammonia production rates of 11.9 and 1.3 x 10(-17) mol/cell.s, respectively. Compared with other monitoring technologies, this technology has many advantages for spaceflights and stand-alone units; for instance, calibration can be performed at one time and then applied in a reagentless, low-maintenance way at a later time. The resulting concentration information can be incorporated into closed-loop control schemes, thereby leading to better in vitro models of in vivo behavior.

Prostatic Neoplasms - metabolism

Ammonia - analysis

Ammonia - metabolism

Humans

Lactic Acid - metabolism

Glutamine - metabolism

Male

Glutamine - analysis

Spectroscopy, Near-Infrared

Bioreactors

Perfusion

Cell Line, Tumor

Glucose - metabolism

Cell Culture Techniques

Glucose - analysis

Lactic Acid - analysis

Details

Title: Subtitle: On-line monitoring of human prostate cancer cells in a perfusion rotating wall vessel by near-infrared spectroscopy
Creators: Martin H Rhiel - Department of Chemical and Biochemical Engineering, 4133 Seamens Center, University of Iowa, Iowa City, IA 52242, USA
Michael B Cohen
Mark A Arnold
David W Murhammer
Resource Type: Journal article
Publication Details: Biotechnology and bioengineering, Vol.86(7), pp.852-861
DOI: 10.1002/bit.10834
PMID: 15162462
NLM abbreviation: Biotechnol Bioeng
ISSN: 0006-3592
eISSN: 1097-0290
Publisher: United States
Language: English
Date published: 06/30/2004
Academic Unit: Pathology; Center for Biocatalysis and Bioprocessing; Chemistry; Chemical and Biochemical Engineering
Record Identifier: 9984003942602771

Metrics

14 Record Views

22 Times Cited - Web of Science